تأثیر تمرین مقاومتی و مکمل عصاره شکلات تلخ بر سطوح پلاسمایی مایوژنین سالمندان

نویسندگان

چکیده

مقدمه و هدف: تمرین ورزشی و تغذیه از جمله راهبرد­های غیردارویی کنترل­ کننده کاهش توده عضلانی ناشی از سالمندی در سالمندان می‌باشند. هدف از پژوهش حاضر بررسی تأثیر هشت هفته تمرین مقاومتی و مصرف مکمل عصاره شکلات تلخ بر میزان مایوژنین سرمی، جهت بررسی آثار مایوژنیکی آن در سالمندان بود.
مواد و روش‌ها: بدین منظور تعداد 36 سالمند (65-85 سال و بطور مساوی زن و مرد) در مرکز خانه سالمندان انتخاب و پس از مراحل اخذ مجوزها، غربالگری‌ و پرکردن فرم رضایت‌نامه به صورت تصادفی به چهار گروه: تمرین مقاومتی (10=n)، مکمل (10=n)، ترکیبی (10=n) و کنترل (6=n) تقسیم شدند. گروه تمرین و ترکیبی به مدت هشت هفته تمرین‌های مقاومتی منتخب را سه جلسه 60 دقیقه‌ای در هفته انجام دادند. گروه مکمل و ترکیبی نیز روزانه یک عدد قرص 500 میلی‌گرمی عصاره شکلات تلخ مصرف کردند. گروه کنترل در این دوره هیچ‌گونه تمرین و مصرف مکملی نداشتند. 48 ساعت قبل و پس از دوره مطالعه 6 سی­ سی نمونه خونی از ورید بازویی آزمودنی‌ها جهت بررسی میزان مایوژنین سرم گرفته و از آزمون آماری آنووا یک­راهه و تی­وابسته جهت تجزیه و تحلیل داده ­ها استفاده شد (0.05>P < span lang="FA">).
یافته ها: نتایج نشان داد میزان مایوژنین در هر سه گروه در داخل و بین گروه ها تفاوت معنی­ داری نداشت (0.05<P < span lang="FA">). هرچند حداکثر قدرت پرس پا و سینه در گرو‌ه ­های تمرین (0.03=P < span lang="FA">) و ترکیبی (P=0.01) در مقایسه با گروه کنترل به صورت معناداری افزایش داشت.
بحث و نتیجه‌گیری: تمرین مقاومتی و مصرف مکمل عصاره شکلات تلخ با­وجود افزایش معنادار حداکثر قدرت عضلانی سینه و پا، تأثیر معنا‌داری بر افزایش عامل تنظیمی مایوژنین ندارد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of resistance training and dark chocolate supplement on plasma myogenin level in elderly

نویسندگان [English]

  • Yasaman honarmandnasab
  • abbasali gaeini
  • mohammadreza kordi
چکیده [English]

Introduction and purpose: Exercise training and nutrition are non-pharmacological strategies which can reduce the sarcopenia-induced muscle atrophy in the older adults. The purpose of this study was to investigate the effect of eight weeks resistance training and dark chocolate supplement on elderly blood myogenin.
Materials and Methods: For this purpose, 36 elderly (65-85 years old, equally men and women) were selected at nursing homes center of Qazvin province. After obtaining permits, screening and filling the random satisfaction form were divided into four groups: resistance training (n=10), supplement (n=10), combination (n=10) and control (n= 6). The training group and the combined group performed the chosen protocol for eight week, 60 minutes each day. The supplement and combination group daily took one 500 mg/day tablet of dark chocolate supplement. The control group did not have any exercise and supplements in this period. 48 hours before and after the study period, 6 cc blood samples were taken from the venous vein of the subjects to determine the plasma myogenin level and ANOVA and T-test were used to analyze the data
(P>0.05).
Results: The results of this study showed that the level of myogenin doesn’t have significantly increase in all three groups (P>0.05). However, the maximum strength of leg and chest press in the training (P=0.03) and combined (P=0.01) groups compared to control group significantly increased.
Discussion and Conclusion: Resistance training and dark chocolate supplement despite the significant increase in maximum muscle strength of the chest and legs, doesn’t have significant positive effect on the increased myogenin regulation.

کلیدواژه‌ها [English]

  • aging
  • Myogenin
  • resistance training
  • sarcopenia
  • Dark chocolate
.1 Watanabe Y, Madarame H, Ogasawara R, Nakazato K, Ishii N. Effect of very low‐intensity resistance training with slow movement on muscle size and strength in healthy older adults. Clinical physiology and functional imaging. 2014;34(6):463-70. .2 Berg KO, Wood-Dauphinee SL, Williams JI, Maki B. Measuring balance in the elderly: validation of an instrument. Canadian journal of public health= Revue canadienne de sante publique. 1991;83:S7-11. .3 Joshua AM, D'Souza V, Unnikrishnan B, Mithra P, Kamath A, Acharya V, et al. Effectiveness of progressive resistance strength training versus traditional balance exercise in improving balance among the elderly-a randomised controlled trial. J Clin Diagn Res. 2014;8(3):98-102. .4 Gonzalez AM, Mangine GT, Fragala MS, Stout JR, Beyer KS, Bohner JD, et al. Resistance training improves single leg stance performance in older adults. Aging clinical and experimental research. 2014;26(1):89-92. .5 Fanzani A, Conraads VM, Penna F, Martinet W. Molecular and cellular mechanisms of skeletal muscle atrophy: an update. Journal of cachexia, sarcopenia and muscle. 2012;3(3):163-79. .6 Jiménez JB, Lluch GL, Martínez IS, Muro-Jiménez A, Bies ER, Navas P. Sarcopenia: implications of physical exercise in its pathophysiology, prevention and treatment. Revista andaluza de medicina del deporte. 2011 (4):158-66. .7 Ruiz JR, Sui X, Lobelo F, Morrow JR, Jackson AW, Sjöström M, et al. Association between muscular strength and mortality in men: prospective cohort study. Bmj. 2008;337:a439. .8 White Z, Terrill J, White RB, McMahon C, Sheard P, Grounds MD, et al. Voluntary resistance wheel exercise from mid-life prevents sarcopenia and increases markers of mitochondrial function and autophagy in muscles of old male and female C57BL/6J mice. Skeletal Muscle. 2016;6(1):1-21. .9 Harri TLTSPCKBDRTTB. Sarcopenia: etiology, clinical consequences, intervention, and assessment. Osteoporos Int. 2010 (21):543–59. .10 Peter J. Abernethy RT, Albert W. Taylor Acute and Chronic Responses of Skeletal Muscle to Endurance and Sprint Exercise. 1990;10(6):365-89. .11 Davidsen PK, Gallagher IJ, Hartman JW, Tarnopolsky MA, Dela F, Helge JW, et al. High responders to resistance exercise training demonstrate differential regulation of skeletal muscle microRNA expression. Journal of applied physiology. 2011. .12 Luginbuhl A, Dudley G, Staron R. Fiber type changes in rat skeletal muscle after intense interval training. Histochemistry. 1984;81(1):55-8. .13 Porter MM. Resistance training recommendations for older adults. Topics in Geriatric Rehabilitation. 2000;15(3):60-9. .14 J. de D. Beas-Jiménez GL-L, I. Sánchez-Martínez , A. Muro-Jiménez , E. Rodríguez-Bies and P. Navas. Sarcopenia: implications of physical exercise in its pathophysiology, prevention and treatmen. Rev Andal Med Deporte. 2011;4(4):158-66. .15 Buitrago-Lopez A, Sanderson J, Johnson L, Warnakula S, Wood A, Di Angelantonio E, et al. Chocolate consumption and cardiometabolic disorders: systematic review and meta-analysis. bmj. 2011;343:d4488. .16 Munguia L, Ramirez-Sanchez I, Meaney E, Villarreal F, Ceballos G, Najera N. Flavonoids from dark chocolate and (-)-epicatechin ameliorate high-fat diet-induced decreases in mobility and muscle damage in aging mice. Food bioscience. 2020 Oct;37. PubMed PMID: 32953444. Pubmed Central PMCID: PMC7497789. Epub 2020/09/22. eng. .17 Taub PR, Ramirez-Sanchez I, Patel M, Higginbotham E, Moreno-Ulloa A, Román-Pintos LM, et al. Beneficial effects of dark chocolate on exercise capacity in sedentary subjects: underlying mechanisms. A double blind, randomized, placebo controlled trial. Food & function. 2016;7(9):3686-93. .18 Alway SE, Myers MJ, Mohamed JS. Regulation of satellite cell function in sarcopenia. Frontiers in aging neuroscience. 2016;6. .19 Lee SJ, Leem YE, Go GY, Choi Y, Song YJ, Kim I, et al. Epicatechin elicits MyoD-dependent myoblast differentiation and myogenic conversion of fibroblasts. PloS one. 2017;12(4):e0175271. PubMed PMID: 28384253. Pubmed Central PMCID: PMC5383328. Epub 2017/04/07. eng. .20 Mackey A, Holm L, Reitelseder S, Pedersen T, Doessing S, Kadi F, et al. Myogenic response of human skeletal muscle to 12 weeks of resistance training at light loading intensity. Scandinavian journal of medicine & science in sports. 2011;21(6):773-82. .21 Sepulveda PV, Lamon S, Hagg A, Thomson RE, Winbanks CE, Qian H, et al. Evaluation of follistatin as a therapeutic in models of skeletal muscle atrophy associated with denervation and tenotomy. Scientific reports. 2015;5(1):1-11. .22 Crameri RM, Langberg H, Magnusson P, Jensen CH, Schrøder HD, Olesen JL, et al. Changes in satellite cells in human skeletal muscle after a single bout of high intensity exercise. The Journal of physiology. 2004;558(1):333-40. .23 Mero A, Hulmi J, Salmijärvi H, Katajavuori M, Haverinen M, Holviala J, et al. Resistance training induced increase in muscle fiber size in young and older men. European journal of applied physiology. 2013;113(3):641-50. .24 Bamman MM, Ronald C. Ragan, Jeong-su Kim, James M. Cross, Vernishia J. Hill, S. Craig Tuggle, and Richard M. Allman. Myogenic protein expression before and after resistance loading in 26- and 64-yr-old men and women. J Appl Physiol. 2004 (97):1329–37. .25 Jeong-su Kim DJK, John K. Petrella, James M. Cross, and Marcas M. Bamman. Resting and load-induced levels of myogenic gene transcripts differ between older adults with demonstrable sarcopenia and young men and women. J Appl Physiol. 2005 (99):2149–58. .26 Aguiar A, Vechetti-Júnior I, de Souza RA, Castan E, Milanezi-Aguiar R, Padovani C, et al. Myogenin, MyoD and IGF-I regulate muscle mass but not fiber-type conversion during resistance training in rats. International journal of sports medicine. 2013;34(04):293-301. .27 Gutierrez-Salmean G, Ciaraldi TP, Nogueira L, Barboza J, Taub PR, Hogan MC, et al. Effects of (−)-epicatechin on molecular modulators of skeletal muscle growth and differentiation. The Journal of nutritional biochemistry. 2014;25(1):91-4. .28 Ota N, Soga S, Haramizu S, Yokoi Y, Hase T, Murase T. Tea catechins prevent contractile dysfunction in unloaded murine soleus muscle: a pilot study. Nutrition. 2011;27(9):955-9. .29 Unhjem RJ. Impact of strength training on the aging neuromuscular system. 2017. .30 Kadi F, Johansson F, Johansson R, Sjöström M, Henriksson J. Effects of one bout of endurance exercise on the expression of myogenin in human quadriceps muscle. Histochemistry and cell biology. 2004;121(4):329-34. .31 Drummond MJ, Conlee RK, Mack GW, Sudweeks S, Schaalje GB, Parcell AC. Myogenic regulatory factor response to resistance exercise volume in skeletal muscle. European journal of applied physiology. 2010;108(4):771-8. .32 Kosek DJ, Kim J-s, Petrella JK, Cross JM, Bamman MM. Efficacy of 3 days/wk resistance training on myofiber hypertrophy and myogenic mechanisms in young vs. older adults. Journal of applied physiology. 2006;101(2):531-44. .33 Mathers JL, Farnfield MM, Garnham AP, Caldow MK, Cameron‐Smith D, Peake JM. Early inflammatory and myogenic responses to resistance exercise in the elderly. Muscle & nerve. 2012;46(3):407-12. .34 White TA, LeBrasseur NK. Myostatin and sarcopenia: opportunities and challenges-a mini-review. Gerontology. 2014;60(4):289-93.