ورزش و علوم زیست حرکتی

ورزش و علوم زیست حرکتی

بررسی تغییرات آنژیوژنز، هایپوکسی بافتی و جریان یونی کلسیم بافت قلبی رت های نابالغ، بالغ و پیر متعاقب تمرین ترکیبی

نوع مقاله : مقاله پژوهشی

نویسندگان
1 استاد فیزیولوژی ورزشی، دانشگاه گیلان، رشت، ایران
2 دانشجوی دکتری تربیت بدنی و علوم ورزشی، دانشگاه گیلان، رشت، ایران
3 استادیار، گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران
چکیده
مقدمه و هدف: در سطح سلولی تاثیرات حفاظت قلبی ناشی از ورزش بر بافت قلب در سنین محتلف متفاوت است. هدف از مطالعه حاضر بررسی تغییرات آنژیوژنز (VEGF)، هایپوکسی بافتی (HIF-1) و جریان یونی کلسیم (Calcineurin) بافت قلبی رت ­های نابالغ، بالغ و پیر متعاقب تمرین ترکیبی م ی­باشد.
مواد و روش ­ها: تعداد 30 سر رت نر نژاد ویستار در سه رده سنی 2 هفتگی، 8 هفتگی و 24 ماهگی تهیه (هر رده سنی 10 سر) و هر رده سنی به دو گروه کنترل و تمرین تقسیم شدند (هر گروه 5 سر). در گروه تمرین ترکیبی حیوانات به صورت یک روز در میان برنامه تمرینی مقاومتی یا هوازی را به مدت 6 هفته انجام می­ دادند. میزان اضافه بار در برنامه تمرین مقاومتی بر اساس وزن بدن حیوانات از 30% در هفته اول به 120% وزن بدن در هفته آخر تعیین شد. برای تمرین هوازی نیز شدت تمرین از 50 درصد حداکثر سرعت در هفته اول به 80 درصد در هفته آخر رسید. پس از 6 هفته تمرین، حیوانات قربانی و بافت قلب برای اندازه­ گیری فاکتورهای VEGF، HIF-1 و Calcineurin با روش RTPCr جدا گردید. برای آنالیز داده‌ها از روش آماری تحلیل واریانس یک­ راهه با معناداری 0.05>P استفاده شد.
یافته­ ها: گروه تمرین 8 هفتگی مقادیر بالاتری در بیان ژن VEGF بافت قلب، نسبت به گروه کنترل خود (کنترل 8 هفتگی) نشان داد (0.02=P). با این وجود، بین گروه­ های تمرین در سه رده در بیان ژن VEGF تفاوت معنی­ داری وجود نداشت (0.05<P). همچنین، بین گروه­ های کنترل و تمرین در هر رده سنی و بین گروه­ های تمرین در سه رده در بیان ژن HIF-1 و Calcineurin قلبی تفاوت معنی­ داری وجود نداشت (0.05<P). 
بحث و نتیجه ­گیری: انجام تمرین ورزشی در سنین جوانی تاثیرات مطلوب­ تر سلولی (به ویژه تنظیم مثبت فاکتور رگ ­زایی) بر جای می­ گذارد. لذا برای داشتن یک دوره سالمندی با کیفیت پیشنهاد می­ شود از سنین جوانی تمرینات مقاومتی- هوازی منظم انجام شود.
 
کلیدواژه‌ها

عنوان مقاله English

Investigating changes in angiogenesis, tissue hypoxia and Calcineurin of cardiac tissue at immature, mature and old rats following combined training

نویسندگان English

Bahman Mirzaei 1
Hossein Ghavami Amin 2
Mohammad Reza Fadaei Chafy 3
1 Professor of Exercise Physiology, University Of Guilan, Rasht, Iran
2 PhD Student of Exercise Physiology, University Of Guilan, Rasht, Iran
3 Assistant Professor, Department of Physical Education and Sport Science, Faculty of Humanities, Rasht Branch, Islamic Azad University, Rasht, Iran.
چکیده English

Introduction and Purpose: At the cellular level, the cardioprotective effects of exercise on the heart tissue are different at various ages. The aim of this study is to investigate the changes in angiogenesis (VEGF), tissue hypoxia (HIF-1) and calcium ion flow (Calcineurin) in the heart tissue of immature, mature and old rats following combined training.
Materials and Methods: Thirty male Wistar rats divided in three age groups of 2 weeks, 8 weeks and 24 months (10 each group) and each age group was divided into two control and exercise training groups (5 each group). In the combined training group, animals performed one day of resistance training and one day of aerobic training for 6 weeks. The amount of overload in the resistance training program was determined based on the body weight of the animals from 30% in the first week to 120% body weight in the last week. For aerobic training, the intensity of training increased from 50% of maximum speed in the first week to 80% in the last week. After 6 weeks of training, sacrificed animals and heart tissue were removed to measure VEGF, HIF-1 and Calcineurin factors by RTPCr method. To analyze data, the statistical method of one-way analysis of variance was used with significance P≤0.05. 
Results: The group of exercise training (8-week) showed higher levels of VEGF gene expression in heart tissue compare to the control group (8-week control) (P=0.020). However, there was no significant difference in VEGF gene expression between training groups in three age groups (P>0.05). Also, there was no significant difference between the control and training groups in each age group and between the training groups in the three age in the expression of cardiac HIF-1 and Calcineurin gene (P>0.05).
Discussion and Conclusion: Exercising at young age has more favorable cellular effects (especially the positive regulation of angiogenesis factor). Therefore, in order to have a good aging period, it was suggested to do regular resistance-aerobic exercises from a young age.

کلیدواژه‌ها English

Combined Training
Angiogenesis
Hypoxia
Calcium
Heart
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