ورزش و علوم زیست حرکتی

ورزش و علوم زیست حرکتی

مقایسه اثر تمرین تناوبی شدید با و بدون مصرف عصاره خرفه بر سطح سرمی MDA و TAC رت های مبتلا به کبد چرب غیرالکلی

نوع مقاله : مقاله پژوهشی

نویسندگان
1 دانشجوی دکتری، گروه تربیت بدنی، واحد نیشابور، دانشگاه آزاد اسلامی، نیشابور، ایران
2 استادیار، گروه تربیت بدنی، واحد نیشابور، دانشگاه آزاد اسلامی، نیشابور، ایران
چکیده
مقدمه و هدف: استرس اکسایشی و آسیب ­های اکسایشی متعاقب آن، یکی از اصلی ترین عوامل پیشرفت بیماری کبدچرب غیرالکلی (NAFLD) می باشد. هدف از تحقیق حاضر مقایسه اثر تمرین تناوبی شدید (HIIT) با و بدون مصرف عصاره خرفه بر سطح سرمی مالون دی آلدئید (MDA) و ظرفیت آنتی اکسیدانی تام (TAC) در رت ­های مبتلا به NAFLD بود.
مواد و روش­ ها: 25 سر رت نر بالغ نژاد ویستار بطور تصادفی انتخاب و در 5 گروه کنترل سالم، کنترل کبدچرب، عصاره خرفه، تمرین HIIT و تمرین+عصاره خرفه قرار داده شدند. جهت القایNAFLD، رت­ها بمدت 12 هفته تحت رژیم غذایی پُرچرب قرار گرفتند. عصاره خرفه با دوز 400 mg/kg به گروه­ های مربوطه خورانده شد. پروتکل تمرینیHIIT بمدت 8 هفته، 5 جلسه در هفته با 7 تکرار یک دقیقه­ای باشدت ۹۰ درصد سرعت بیشینه انجام شد که با تناوب­های استراحت فعال شامل 2 دقیقه دویدن با شدت ۲۰ درصد بیشینه همراه بود. برای مقایسه بین گروه ­ها از آزمون آنالیز واریانس یک طرفه و آزمون تعقیبی توکی در سطح معناداری 0.05>P استفاده شد.
یافته ­ها: سطح سرمی MDA در گروه کنترل کبد چرب به طور معنی داری نسبت به کنترل سالم بالاتر بود (0.034=P). سطح این شاخص در گروه عصاره و تمرین+عصاره بطور معناداری نسبت به گروه کنترل کبدچرب پایین ­تر بود (مقادیر P بترتیب 0.021 و 0.049). اما سطح سرمی MDA در گروه تمرین نسبت به کنترل کبد چرب، تفاوت معناداری نداشت (0.08=P). سطح سرمی TAC در گروه عصاره نسبت به گروه کنترل سالم و کنترل کبد چرب بطور معناداری بالاتر بود (مقادیر P بترتیب 0.044 و 0.001). سطح این شاخص در گروه تمرین+عصاره نسبت به گروه کنترل کبد چرب بطور معناداری بالاتر بود (0.003=P).
بحث و نتیجه گیری: بنظر می ­رسد تمرین تناوبی شدید طولانی مدت و مصرف عصاره خرفه از طریق کاهش پراکسیداسیون لیپیدی و بهبود عوامل آنتی اکسیدانی در رت­ های NAFLD، می­ تواند نقش مهمی را در کنترل پیشرفت این بیماری برعهده داشته باشند.
کلیدواژه‌ها

عنوان مقاله English

Comparison the Effect of High Intensity Interval Training with and without Portulaca Oleracea Extract on Serum Level of MDA and TAC in Rats with Non-Alcoholic Fatty Liver Disease

نویسندگان English

Mahdi Javadikia 1
Ameneh Barjaste yazdi 2
Rambod Khajei 2
Mohamad reza Hosein abadi 2
1 PhD Candidate, Department of Physical Education, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran
2 Assistant Professor, Department of Physical Education, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran
چکیده English

Introduction and purpose:  Oxidative stress and subsequent oxidative damage is one of the main factors in the development of non-alcoholic fatty liver disease (NAFLD). The aim of present study was to investigate comparison the effect of High Intensity Interval Training (HIIT) with and without Portulaca Oleracea Extract on Serum Level of Malondialdehyde (MDA) and Total Antioxidant Capacity (TAC) in Rats with (NAFLD).
Materials and methods: Twenty five male Wistar rats were randomly divided into five groups: healthy control, fatty liver control, Portulaca Oleracea, HIIT and HIIT+ Portulaca Oleracea. To induce NAFLD, the rats were fed a high-fat diet for 12 weeks. Portulaca Oleracea supplement at 400 mg/kg was given to the experimental groups. HIIT was performed for 8 weeks, 5 sessions per week with 7 repetitions of 1 minute at 90% maximum speed accompanied by active rest intervals including 2 minutes of running at 20% mmaximum speed. To analyze the data, one-way analysis of variance and Tukey's post hoc test were used at the significance level of p<0.05.
Results: Serum Level of MDA in fatty liver control was higher than healthy control group (P=0.034). Level of this index in Portulaca Oleracea Extract and HIIT+ Portulaca Oleracea groups were significantly lower than fatty liver control group (P values 0.021 and 0.049 respectively). But No significant difference was observed in the serum level of MDA in the HIIT group compared to the fatty liver control group (P=0.08). Serum level of TAC were significantly higher in Portulaca Oleracea Extract than healthy control and fatty liver control groups (P values 0.044 and 0.001 respectively). Level of this index in HIIT+ Portulaca Oleracea Extract group was significantly higher than fatty liver control group (P=0.003).
Discussion and conclusion: It seems that long term HIIT and Consumption of Portulaca Oleracea extract by reducing lipid peroxidation and improving antioxidant factors in NAFLD subjects, can play an important role in controlling the progress of this disease.

کلیدواژه‌ها English

High Intensity Interval Training
Portulaca Oleracea extract
Malondialdehyde
Total Antioxidant Capacity
Non-Alcoholic Fatty Liver
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