ورزش و علوم زیست حرکتی

ورزش و علوم زیست حرکتی

تأثیر تمرین هوازی بر بیان ژن mir-195 در بافت قلب موش‌های صحرایی مبتلا به دیابت نوع دو

نوع مقاله : مقاله پژوهشی

نویسندگان
الهه پیرعلائی 1
بدرخان رشوان اسماعیل 2
غلامرضا حمیدیان 3
1 استادیار، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تبریز، تبریز، ایران.
2 دانشجوی دکتری، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تبریز، تبریز، ایران.
3 دانشیار بافت شناسی مقایسه‌ای، گروه علوم پایه، دانشکده دامپزشکی، دانشگاه تبریز، تبریز، ایران.
10.22034/sbs.2024.460274.1101
چکیده
مقدمه و هدف: در سال‌های اخیر رابطه بسیار نزدیکی بین miRNA و بیماری‌های دیابت و قلبی عروقی پیدا شده است. بنابراین، این تحقیق با هدف بررسی تاثیر هشت هفته تمرین هوازی بر بیان ژنmir-195 در بافت قلب موش‌های صحرایی مبتلا به دیابت نوع دو انجام شد.
مواد و روش­‌ها: برای این منظور، 18 سر موش صحرایی نر نژاد ویستار (با میانگین وزنی 240 گرم و میانگین سن 8 هفته‌ای) بصورت تصادفی به سه گروه 1) کنترل سالم، 2) کنترل دیابتی، و 3) تمرین دیابتی تقسیم شدند. برای القاء دیابت نوع دو، ابتدا یک رژیم­‌غذایی پرچرب (60 درصد) به مدت دو هفته و سپس تزریق استرپتوزوسین در حالت ناشتا صورت گرفت. پروتکل تمرین دویدن روی تردمیل پنج روز در هفته با رعایت اصل اضافه بار در هفته اول با سرعت 10-5 متر در دقیقه به مدت 10-15 دقیقه و در هفته هشتم با سرعت 18-24 متر در دقیقه به مدت 60 دقیقه به دست آمد. برای بررسی بیان ژن miR-195 از روش Real-time PCR استفاده شد. از آزمون‌­های تحلیل واریانس یک‌راهه و تعقیبی توکی، در سطح معنی­داری 0.05≥P استفاده شد.
یافته‌­ها: هشت هفته ابتلاء به T2DM منجر به افزایش معنی‌­دار در گلوکز و بیان ژن miR-195 شد (0.05≥P). با این حال هشت هفته تمرین هوازی منجر به کاهش معنی­‌دار در گلوکز و بیان ژن miR-195  نسبت به گروه کنترل دیابتی شد (0.05≥P).
نتیجه‌گیری: نتایج ما نشان داد که ورزش به ویژه تمرینات هوازی یک استراتژی مناسب برای کاهش miR-195 و بهبود عملکرد میوکارد در موش‌های دیابتی است.
کلیدواژه‌ها
تمرین هوازی
ژن miR-195
بافت قلب
موش‌های صحرایی
دیابت نوع دو

موضوعات

عنوان مقاله English

The effect of aerobic training on the mir-195 gene expression in heart tissue of type 2 diabetic rats

نویسندگان English

Elaheh Piralaiy 1
Badrkhan Rashwan Ismael 2
Gholamreza Hamidian 3
1 Assistant Professor, Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, Tabriz University, Tabriz, Iran.
2 Ph.D Candidate, Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, Tabriz University, Tabriz, Iran.
3 3. Associate Professor in Comparative Histology, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
چکیده English

Introduction and Purpose: In recent years, a strong association has been identified between miRNA and diseases such as diabetes and cardiovascular disorders. Therefore, this study aimed to investigate the effect of eight weeks of aerobic exercise on the expression of the mir-195 gene in the heart tissue of rats with type 2 diabetes.
Materials and methods: For this purpose, 18 male Wistar rats (average weight: 240 g, average age: 8 weeks) were randomly assigned to three groups: (1) healthy control, (2) diabetic control, and (3) diabetic exercise. To induce type 2 diabetes, the rats were first fed a high-fat diet (60%) for two weeks, followed by fasting streptozotocin injection. The treadmill running protocol was performed five days a week, following the principle of progressive overload. In the first week, the exercise was conducted at a speed of 5–10 m/min for 10–15 minutes, and by the eighth week, it was increased to 18–24 m/min for 60 minutes. Gene expression of miR-195 was assessed using real-time PCR. One-way ANOVA and Tukey’s post hoc test were used for statistical analysis at a significance level of P≤0.05.
Results: Eight weeks of T2DM led to a significant increase in glucose levels and miR-195 gene expression (P≤0.05). However, eight weeks of aerobic exercise significantly reduced glucose levels and miR-195 gene expression compared to the diabetic control group (P≤0.05).
Discussion and Conclusion: Our results suggest that exercise, particularly aerobic training, is an effective strategy for reducing miR-195 expression and improving myocardial function in diabetic rats.

کلیدواژه‌ها English

Aerobic training
mir-195 gene
Heart tissue
Rats
Type 2 diabetes
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ورزش و علوم زیست حرکتی
دوره 16، شماره 32
اسفند 1403
صفحه 39-49

  • تاریخ دریافت 12 خرداد 1403
  • تاریخ بازنگری 24 شهریور 1403
  • تاریخ پذیرش 07 مهر 1403