ورزش و علوم زیست حرکتی

ورزش و علوم زیست حرکتی

بررسی تغییرات BDNF و آیریزین در عملکرد شناختی به دنبال 6 هفته تمرین تناوبی شنا با شدت بالا در هیپوکمپ موش‌های صحرایی نر بالغ ویستار

نوع مقاله : مقاله پژوهشی

نویسندگان
1 استادیار، گروه تربیت بدنی، دانشکده علوم اجتماعی، دانشگاه بین المللی امام خمینی، قزوین، ایران.
2 استادیار، گروه تربیت بدنی، دانشگاه صنعتی امیرکبیر، تهران، ایران.
3 استادیار، گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه هرمزگان، هرمزگان، ایران
4 استادیار، گروه علوم پایه، دانشگاه صنعتی همدان، ایران.
چکیده
مقدمه و هدف: آیریزین و عامل نوروتروفیک مغزی به­ عنوان شاخص­های مولکولی درگیر در عملکرد شناختی می­ باشند. پژوهش حاضر به دنبال بررسی اثر شنا با شدت بالا بر تغییرات این دو عامل و عملکرد شناختی می­ باشد.
مواد و روش ­ها: روش پژوهش از نوع تجربی بود. 20 سرموش صحرایی نر ویستار (سن 8 هفته، وزن 30 ±250 گرم) پس از دو هفته آشناسازی با محیط استاندارد حیوانات در آزمایشگاه و پروتکل تمرین، به­ طور تصادفی به دو گروه تمرین و کنترل تقسیم شدند. گروه تمرین در شش هفته تمرین شنا با شدت بالا شرکت کرد و 48 ساعت پس از آخرین جلسه­ تمرین، موش ­ها قربانی و بافت هیپوکمپ آن­ها  برداشته شد. برای سنجش بیان ژن آیریزین و BDNF از روش real-time PCR و برای ارزیابی عملکرد شناختی از آزمون آبی موریس استفاده شد. برای تحلیل یافته­ های بیان ژن از آزمون تی مستقل و برای عملکرد شناختی از آزمون کوواریانس در سطح معناداری 0.05>P استفاده شد.
یافته ­ها: نتایج نشان داد، شش هفته شنا با شدت بالا موجب افزایش معنادار سطوح آیریزین، BDNF (P=0.001) و بهبود عملکرد شناختی (P=0.001) گروه تمرین نسبت به گروه کنترل شد.
بحث و نتیجه‌گیری: سطوح آیریزین و BDNF هیپوکمپ در پاسخ به شش هفته تمرینات شنا با شدت بالا به­ طور معناداری افزایش می ­یابد و این افزایش با بهبود عمکرد آزمون شناختی ماز آبی همراستا بود. از این‌رو به ­نظر می­ رسد بخشی از بهبود عملکرد شناختی متعاقب پروتکل تمرین با تغییرات افزایشی آیریزین و BDNF سلول­ های مغزی رخ داده باشد.
کلیدواژه‌ها

عنوان مقاله English

Evaluation of BDNF and Irisin changes involved in cognitive performance after 6 weeks of intermittent high-intensity swimming training in the hippocampus of adult male Wistar rats

نویسندگان English

fateme mirakhori 1
zahra mirakhori 2
elham Shahabpour 3
saeed Daneshyar 4
1 Assistant Professor, Department of Physical Education, Faculty of Social Sciences, International University of Emam Khomeini, Ghazvin, Iran.
2 Assistant Professor, Department of Physical Education, Amirkabir University of Technology, Tehran, Iran.
3 Assistant Professor, Department of Sport Sciences, Faculty of Humanities Sciences, University of Hormozgan, Hormozgan, Iran.
4 Assistant Professor, Department of Basic Sciences, Hamedan University of Technology, Hamedan, Iran.
چکیده English

Introduction and Purpose: Irisin and brain neurotrophic factor are involved in cognitive function as molecular indicators. The present study investigated the effect of high-intensity swimming on the changes of these two factors and cognitive performance.
Materials and Methods: The research method was experimental. 20 male Wistar rats (age 8 weeks, weight 250 ± 30 grams) were randomly divided into training and control groups after two weeks of familiarization with the standard environment of the animals in the laboratory and the training protocol. The training group participated in six weeks of high-intensity swimming training, and 48 hours after the last training session, the mice were sacrificed and their hippocampus tissue was removed. Real-time PCR method was used to measure irisin and BDNF gene expression, and Morris water test was used to evaluate cognitive function. Independent t-test was used to analyze gene expression findings and covariance test was used for cognitive function at a significance level of P<0.05.
Results: The results showed that six weeks of high-intensity swimming significantly increased the levels of irisin, BDNF (P=0.001) and improved cognitive performance (P=0.008) in the training group compared to the control group.
Discussion and Conclusion: Irisin and BDNF levels of the hippocampus significantly increase in response to six weeks of high-intensity swimming training, and this increase was in line with the improvement of the cognitive test of water maze. Therefore, it seems that a part of the cognitive performance improvement following the training protocol occurred with the incremental changes of irisin and BDNF in brain cells.

کلیدواژه‌ها English

Irisin
cognitive function
retardation and hippocampus
Smiley-Oyen AL LK, Francois SJ, Kohut ML, Ekkekakis P. Exercise, fitness, and neurocognitive function in older adults:
The “selective improvement” and “cardiovascular fitness” hypotheses. Annals of Behavioral Medicine. 2008;36(3):280–
91. doi: 10.1007/s12160-008-9064-5.
2. Erickson KI VM, Prakash RS, Basak C, Szabo A, Chaddock L, KimJS & et al. Exercise training increases size of
hippocampus and improves memory. Proceeding of the National Academy of Sciences of the United States of America.
2011;108(7):3017–22. doi: 10.1073/pnas.1015950108.
3. Ahmadiasl NAH, H€anninen O. Effect of exercise on learning, memory and levels of epinephrine in rats’ hippocampus.
Journal of Sports Science and Medicine. 2003;2(3):106–9. doi: 24627662/8753-90760.
4. Muller PTM, Muller NG. Physical exercise as personalized medicine for dementia prevention? Frontiers in Physiology.
2019;10:672. doi: 10.3389/fphys.2019.00672.
5. Clark REBN, Squire LR. Hippocampus and remote spatial memory in rats. Hippocampus. 2005;15(2):260–72.
doi: 10.1002/hipo.20056.
6. Cotman CWCC, Berchtold NC. Exercise: a behavioral intervention to enhance brain health and plasticity. Trends Neurosci. 2005;25(6):295–301. doi: 10.1016/s0166-2236(02)02143-4
7. AkaN M. Brain-derived neurotrophic factor is critically involved in thermal-experience-dependent developmental
plasticity. The Journal of Neuroscience. 2006;26(15):3899-907. doi: 10.1523/JNEUROSCI.0371-06.2006.
8. Rasmussen P BP, Adser H, Pedersen MV, Leick L, Hart E, Secher NH &et al. Evidence for a release of brain-derived
neurotrophic factor from the brain during exercise. Experimental Physiology. 2009;4(10):1062–9.
doi: 10.1113/expphysiol.2009.048512.
9. Bostrom PWJ, Jedrychowski MP, Korde A, Ye L, Lo JC & et al. A PGC1-alpha-dependent myokine that drives brownfat-like development of white fat and thermogenesis. Nature 2012;481:463–8. doi: 10.1038/nature10777.
10. Gur FMT S, Yalcin MH, Girgin A, GencerTarakci B. Immunohistochemical localization of irisin in mole rats
(Spalaxleucodon). Biotech Histochem. 2017;92: 245–51. doi: 10.1080/10520295.2017.1303194.
11. Piya MKH, Sivakumar K, Tripathi G, Voyias PD, James S, Sabico S& et al. The identification of irisin in human
cerebrospinal fluid: Influence of adiposity, metabolic markers, and gestational diabetes. American Journal of Physiology
Endocrinology and Metabolism. 2014(306): 512–8. doi: 10.1152/ajpendo.00308.2013.
12. Wrann CDW JP, Salogiannnis J. Exercise induces hippocampal BDNF through a PGC-1alpha/FNDC5 pathway. Cell
Metabolism. 2013;18:649–59. doi: 10.1016/j.cmet.2013.09.008.
13. Forouzanfar MRF, Ghaedi K, Beheshti S, Tanhaei S, ShoarayeNejati A, JodeiriFarshbaf M & et al. Fndc5 overexpression
facilitated neural differentiation of mouse embryonic stem cells. Cell Biology International. 2015;39:629–37.
doi: 10.1002/cbin.10427.
14. Moon HSDF, Mantzoros, CS. Pharmacological concentrations of irisin increase cell proliferation without influencing
markers of neurite outgrowth and synaptogenesis in mouse H19-7 hippocampal cell lines. Metabolism. 2013;62:1131–6.
doi: 10.1016/j.metabol.2013.04.007.
15. Murawska-Cialowicz EW, Zuwala-Jagiello J, Feito Y, Petr M, Kokstejn J, Stastny P & et al. Effect of HIIT with tabata
protocol on serum irisin, physical performance, and body composition in men. International Journal of Environmental
Research and Public Health. 2020;17:3589. doi: 10.3390/ijerph17103589.
16. Tsuchiya YA, Takamatsu K, Goto K. Resistance exercise induces a greater irisin response than endurance exercise.
Metabolism. 2015;64:1042–50. doi: 10.1016/j.metabol.2015.05.010.
17. Li DJL YH, Yuan HB, Qu LF, Wang P. The novel exercise-induced hormone irisin protects against neuronal injury via
activation of the Akt and ERK1/2 signaling pathways and contributes to the neuroprotection of physical exercise in cerebral
ischemia. Metabolism. 2017;68:31–42. doi: 10.1016/j.metabol.2016.12.003.
18. De Oliveira Bristot VJdBA AC, Cardoso LR, Da Luz Scheffer D, Aguiar AS. The role of PGC-1/UCP2 signaling in the
beneficial effects of physical exercise on the brain. Frontiers in Neuroscience. 2019;13:292. doi: 10.3389/fnins.
2019.00292.
19. Shams S, Rajabi H, Suzuki K. Swimming in cold water upregulates genes involved in thermogenesis and the browning of white adipose tissues. Comparative Biochemistry and Physiology. 2023;Part B 265:110834. doi: 10.1016/j.cbpb.
2023.110834.
20. McNamara RK SR. The neuropharmacological and neurochemical basis of place learning in the Morris water maze. Brain
Research Reviews. 1993;18(1):33-49. doi: 10.1016/0165-0173(93)90006-l.
21. D’Hooge R DDP. Applications of the Morris water maze in the study of learning and memory. Brain Research Reviews.
2001;36(1):60-90. doi: 10.1016/s0165-0173(01)00067-4.
22. N GMSMAHN. Isolation of neural stem and progenitor cells from the adult mouse brain using the neurosphere assay.
Journal of Ardanil university of medical sciences. 2013;11(3):246-58. http://jarums.arums.ac.ir/article-1-174-en.html.
23. Spaniol J. Event-related fMRI studies of episodic encoding and retrieval: Meta-analyses using activation likelihood
estimation. Neuropsychologia. 2009;47:1765–79. doi: 10.1016/j.neuropsychologia.2009.02.028.
24. Bherer LE KI, Liu-Ambrose, T. A Review of the effects of physical activity and exercise on cognitive and brain functions
in older adults. Journal of Aging Research. 2013;2013:1-8. doi: 10.1155/2013/657508.
25. Zahra Mirakhori FM. The effect of regular exercise on cognitive function and irisin expression. sport physiology &
management investigations. 2023;15(3):159-70. [doi:10.22034/SPMI.2023.191101] [In Persian].
26. Petzinger GMF, Holschneider DP, Wood R, Walsh JP, Lund B, Meshul C & Eet al. The role of exercise in facilitating
basal ganglia function in Parkinson’s disease. Neurodegeneativer Disease Management. 2011;1:157-70.
doi: 10.2217/nmt.11.16.
27. Park HP. Neurotrophin regulation of neural circuit development and function. Nature reviews Neuroscience. 2013;14(7-
23). doi: 10.1038/nrn3379.
28. Christopher W. Collins RJS, Matthew W. S. Heesch, and Dustin R. Slivka. The effect of environmental temperature on
exercise-dependent release of brain-derived neurotrophic factor. TEMPERATURE. 2017;4(3):305–13. doi: 10.1080/
23328940.2017.1328304.
29. Wrann CD WJ, Salogiannnis J, Laznik-Bogoslavski D, Wu J, Ma D, Lin JD, Greenberg ME, Spiegelman BM. . Exercise
induces hippocampal BDNF through a PGC-1alpha/ FNDC5 pathway. Cell Metabolism. 2013;18(5):649–59.
doi: 10.1016/j.cmet.2013.09.008.
30. Cotman CWB, Christie LA. Exercise builds brain health: Key roles of growth factor cascades and inflammation. Trends
Neurosci. 2007;30:464–72. doi: 10.1016/j.tins.2007.06.011.
31. Qin WSL, Cao J, Peng Y, Collier L, Wu Y, Creasey G & et al. The central nervous system (CNS)-independent anti-boneresorptive activity of muscle contraction and the underlying molecular and cellular signatures. Journal of Bioogicall
Chemistry. 2013;288:13511–21. doi: 10.1074/jbc.M113.454892.
32. Chang WTL, Tang Y, Ahmad S, Zhang H, Yap PT, Giovanello KS & et al. Brain wide functional networks associated
with anatomically- and functionally-defined hippocampal subfields using ultrahigh-resolution fMRI. Scientific Reports.
2021;11:10835. doi: 10.1038/s41598-021-90364-7.
33. Norris D. Short-term memory and long-term memory are still different. Psychological Bulletin. 2017;143:992–1009.
doi: 10.1037/bul0000108.
34. Hashemi MSG, Salamian A, Karbalaie K, Emadi-Baygi M, Tanhaei S, Nasr-Esfahani MH & et al. Fndc5 knockdown
significantly decreased neural differentiation rate of mouse embryonic stem cells. Neuroscience. 2013;231:296–304.
doi: 10.1016/j.neuroscience.2012.11.041.
35. Choi SHB, Chatila ZK, Lee SW, Pulli B, Clemenson GD, Kim E & et al. Combined adult neurogenesis and BDNF mimic
exercise effects on cognition in an Alzheimer’s mouse model. Science. 2018;36:eaan8821. doi: 10.1126/science.aan8821.
36. Schnyder SHC. Skeletal muscle as an endocrine organ: PGC-1, myokines and exercise. Bone. 2015;80:115–25.
doi: 10.1016/j.bone.2015.02.008.
37. Lourenco MVR, Sudo FK, Drummond C, Assunção N, Vanderborght B, Tovar-Moll F. Matto Cerebrospinal fluid irisin
correlates with amyloid, BDNF, and cognition in Alzheimer’s disease. Dement Diagn Assess Dis Monit. 2020;12:e12034.
doi: 10.1002/dad2.12034.
38. Pesce ILFM, Paolucci T, Grilli A, Patruno A, Agostini F, Bernetti A & et al. From exercise to cognitive performance: role
of irisin. Applied Sciences. 2021;11(7120):1-15. doi: 10.3390/app11157120.

  • تاریخ دریافت 19 فروردین 1403
  • تاریخ بازنگری 03 شهریور 1403
  • تاریخ پذیرش 07 شهریور 1403