ورزش و علوم زیست حرکتی

ورزش و علوم زیست حرکتی

تاثیر تمرین شنای با زمان متفاوت، بر بیان ژن های انتقال معکوس کلسترول بافت کبد موش های نر صحرایی

نوع مقاله : مقاله پژوهشی

نویسندگان
1 استادیار، گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه، دامغان، ایـران.
2 دکترای تخصصی، گروه فیزیولوژی ورزش، دانشکده علوم ورزشی، دانشگاه بوعلی سینا، همدان، ایران
چکیده
مقدمه و هدف: جمع ­آوری کلسترول اضافی از بافت­ های پیرامونی و بازگرداندن آن به کبد به منظور تشکیل صفرا، فرآیند انتقال معکوس کلسترول است، که متاثر از تمرین­ های ورزشی با مدت زمان و شدت مختلف می ­باشد. هدف از پژوهش حاضر بررسی تاثیر تمرین شنا در دو زمان متفاوت بر بیان ژن ­های موثر بر انتقال معکوس کلسترول بافت کبد موش­ های نر بود.
مواد و روش ­ها: مطالعه حاضر از نوع مقطعی کاربردی است، که تعداد 18 سر موش نر نژاد صحرایی (20±210 گرم) به ­صورت تصادفی به سه گروه شش­ تایی کنترل، شنای میان و شنای بلندمدت تقسیم شدند. گروه ­های تمرینی به مدت ده هفته و پنج روز در هفته در آب 2 ±30 درجه سانتی­گراد شنا کردند. در هر جلسه گروه میان ­مدت یک ساعت و گروه بلندمدت سه ساعت شنا کردند. لوب سمت چپ کبد جدا گردید و برای سنجش بیان ژن از روش Real-time PCR توسط مستر میکس Ampliqon و کیت سنتز DNA ساخت دانمارک استفاده شد. تفاوت­ ها با روش تحلیل واریانس یک­ سویه و مقایسه­ گروه ­ها توسط آزمون تعقیبی توکی تعیین گردید (سطح معنی­داری P≤0.05).
یافته ­ها: در گروه بلند مدت، مقادیر بیان ژن­های ABCA1 و ApoA1 در مقایسه با گروه کنترل افزایش معنی ­دار (P=0.04) و ژن ApoB در مقایسه با گروه کنترل (P=0.003) و در مقایسه با گروه میان­ مدت (P=0.009) افزایش معنی ­داری یافت.
بحث و نتیجه­ گیری: به­ نظر می­رسد که اجرای  تمرین بلندمدت در مقایسه با میان ­مدت بر فرآیند انتقال معکوس کلسترول تاثیر بیشتری داشته باشد، بر همین اساس انتخاب زمان تمرین ورزشکاران یا بیماران کبدی تاثیر مهمی بر سطوح کلسترول دارد.
کلیدواژه‌ها

عنوان مقاله English

The effect of swimming training with different time on the expression of cholesterol reverse transport genes in the liver tissue of male Wistar rats

نویسندگان English

Maliheh Ardakanizadeh 1
Leyla Vesaliakbarpour 2
1 Department of Sport Sciences, Faculty of Human Sciences, Damghan University, Damghan, Iran.
2 PhD, Department of Exercise Physiology, Faculty of Sport Sciences, Bu_Ali Sina University, Hamedan, Iran.
چکیده English

Introduction and Purpose: The process of reverse cholesterol transport, which includes the collection of excess cholesterol from the surrounding tissues and its return to the liver for bile formation, is affected by exercise training with different durations and intensities. The purpose of the present study was to investigate the effect of swimming training at two different times on the expression of genes affecting the reverse cholesterol transport in the liver tissue of male rats.
Materials and Methods: This applied cross-sectional study conducted on 18 male rats (210 ± 20 g) randomly divided into three groups of six: control, moderate and long-term swimming. The training groups swam in water at 30 ± 2° C for ten weeks, 5 days per week. In each session, the moderate-term group swam for one hour and the long-term group for three hours. The left lobe of the liver was separated and gene expression was measured using real-time PCR method by Ampliqon Master Mix, and DNA synthesis kit made in Denmark. Differences were determined by One-Way ANOVA, and comparison of groups was determined by Tukey's post hoc test (P≤0.05).
Results: In long-term group, the expression values of ABCA1 and ApoA1 gene were significantly increased compared to the control group (P=0.04), and the ApoB gene was significantly increased compared to the control group (P=0.003), and the moderate-term group (P=0.009).
Discussion and Conclusion: It seems that long-term exercise has a greater effect on the process of reverse cholesterol transport compared to moderate-term exercise. Therefore, choosing the training time for athletes or liver patients can significantly affect cholesterol levels.

کلیدواژه‌ها English

Reverse cholesterol transport
Liver
medium-term exercise
long-term exercise
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  • تاریخ دریافت 23 آبان 1402
  • تاریخ بازنگری 21 مرداد 1403
  • تاریخ پذیرش 09 شهریور 1403