ورزش و علوم زیست حرکتی

ورزش و علوم زیست حرکتی

تاثیر تمرینات استقامتی، مقاومتی و تناوبی شدید بر بیان ژن لیپوکالین پروستاگلاندین D سنتاز، وزن وشاخص لی موش‌های چاق

نوع مقاله : مقاله پژوهشی

نویسندگان
1 دانشجوی دکتری، گروه تربیت بدنی و علوم ورزشی، واحد بجنورد، دانشگاه آزاد اسلامی، بجنورد، ایران.
2 استادیار، گروه تربیت بدنی و علوم ورزشی، واحد بجنورد، دانشگاه آزاد اسلامی، بجنورد، ایران.
چکیده
مقدمه و هدف: چاقی و اضافه وزن یک اختلال سوخت و سازی است که سطوح بافت چربی در این اختلال تاثیرگذار است. لیپوکالین پروستاگلاندین D سنتاز یک آنزیم منحصر به فرد در خانواده لیپوکالین است و نقش مهمی در بیماری‌های متابولیک مانند چاقی دارد. هدف از پژوهش حاضر، تاثیر هشت هفته تمرینات متفاوت بر بیان ژن لیپوکالین پروستاگلاندین D سنتاز، وزن و شاخص لی موش‌های چاق بود.
مواد و روش‌ها: برای این منظور، تعداد 40 سر موش صحرایی نر نژاد ویستار چاق شده با رژیم غذایی پرچرب، به صورت تصادفی به چهار گروه مساوی تمرین استقامتی، مقاومتی، تناوبی شدید و کنترل تقسیم شدند. موش­های گروه‌های تجربی به مدت هشت هفته، هفته­ ای پنج جلسه تمرین استقامتی با شدت 70 تا80 درصد سرعت بیشینه، مقاومتی با شدت 50 تا 120 درصد وزن بدن و تناوبی شدید با شدت 85 تا 90 درصد سرعت بیشینه را انجام دادند و هر هفته 5 درصد به شدت تمرینات اضافه شد. برای اندازه­گیری بیان ژن لیپوکالین پروستاگلاندین D سنتاز از روش Real Time-PCR استفاده شد. از روش آماری تحلیل واریانس یک ­طرفه و آزمون تعقیبی توکی جهت تعیین اختلاف بین­ گروه‌ها در سطح  معناداری 0.05≥P استفاده شد.
یافته‌ها: پس از هشت هفته تمرینات استقامتی، مقاومتی و تناوبی شدید، بیان ژن لیپوکالین پروستاگلاندین D سنتاز (0.001=P) در گروه‌های تمرین نسبت به گروه کنترل به‌طور معنا‌داری افزایش یافت. وزن (0.001=P) و شاخص لی (0.001=P) در گروه‌های تمرین نسبت به گروه کنترل به‌طور معناداری کاهش یافت. بین گروه‌های تمرینی تفاوت معناداری مشاهده نشد (0.05<P).
بحث و نتیجه‌گیری: تمرینات استقامتی، مقاومتی و تناوبی شدید با تأثیر مثبت بر عامل درون‌ سلولی لیپوکالین پروستاگلاندین D سنتاز، کاهش وزن و شاخص لی می‌توانند به‌عنوان راهکار موثر در زمینه افزایش لیپولیز و کاهش اختلالات مرتبط با اضافه وزن و چاقی در نظر گرفته شوند.
کلیدواژه‌ها

عنوان مقاله English

The effect of endurance, resistance and high intensity interval training on lipocalin prostaglandin D synthase gene expression, weight and Lee index of obese rats

نویسندگان English

hosein kavosi hasanabad 1
Sadegh Cheragh Birjandi 2
najmeh rezaeian 2
Ali Yaghoubi 2
1 PhD Student, Department of Physical Education and Sport Sciences, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran.
2 Assistant Professor, Department of Physical Education and Sport Sciences, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran.
چکیده English

Introduction and Purpose: Obesity and overweight is a metabolic disorder that affects the levels of fat tissue in this disorder. Lipocalin prostaglandin D synthase is a unique enzyme in the lipocalin family and plays an important role in metabolic diseases such as obesity. The aim of the present study was the effect of eight weeks of different exercises on lipocalin prostaglandin D synthase gene expression, weight and Li index of obese rats.
Materials and Methods: For this purpose, 40 male Wistar rats, fattened with a high-fat diet, were randomly divided into four equal groups of endurance training, resistance training, intense intermittent training, and control. For eight weeks, the rats of the experimental groups performed five sessions of endurance training with an intensity of 70-80% of the maximum speed, resistance with an intensity of 50-120% of the body weight and intense intervals with an intensity of 85-90% of the maximum speed and Every week, 5% was added to the intensity of the exercises. Real Time-PCR method was used to measure lipocalin prostaglandin D synthase gene expression. The statistical method of one-way analysis of variance and Tukey's post hoc test was used to determine the difference between groups at a significance level of P<0.05.
Results: After eight weeks of intense endurance, resistance and periodic training, lipocalin prostaglandin D synthase gene expression increased significantly (P=0.001) in the training groups compared to the control group. Weight (P=0.001) and Lee's index (P=0.001) decreased significantly in the training groups compared to the control group. No significant difference was observed between training groups (P<0.05).
Discussion and Conclusion: Endurance, resistance and high intense intermittent training with a positive effect on the intracellular agent lipocalin prostaglandin D synthase, weight loss and Lee's index can be considered as an effective solution in the field of increasing lipolysis and reducing disorders related to overweight and obesity.

کلیدواژه‌ها English

Endurance training
Resistance training
High intensity interval training
L-PGDS
obesity
Bagheri T, Cheragh-Birjandi S. Effect of eight weeks of HIIT training on serum levels of C1q/TNF5, insulin resistance,
and fat percentage in overweight women. Journal of Sport and Exercise Physiology. 2018;11(2):75-86. doi:10.48308/
JOEPPA.2019.98940. [In Persian].
2. Wang J, Zhang Q, Xia J, Sun H .Moderate treadmill exercise modulates gut microbiota and improves intestinal barrier in
high-fat-diet-induced obese mice via the AMPK/CDX2 signaling pathway. Diabetes, metabolic syndrome and obesity:
targets and therapy. 2022:209-23. doi:10.2147/DMSO.S346007.
3. Brezinova M, Cajka T, Oseeva M, Stepan M, Dadova K, Rossmeislova L, et al. Exercise training induces insulinsensitizing PAHSAs in adipose tissue of elderly women. Biochimica et Biophysica Acta (BBA)-Molecular and Cell
Biology of Lipids. 2020;1865(2):158576. doi:10.1016/j.bbalip.2019.158576.
4. Zaghlool SB, Halama A, Stephan N, Gudmundsdottir V, Gudnason V, Jennings LL, et al. Metabolic and proteomic
signatures of type 2 diabetes subtypes in an Arab population. Nature communications. 2022;13(1):7121. doi:10.1038/
s41467-022-34754-z.
5. Álvarez-Almazán S, Bello M, Tamay-Cach F, Martínez-Archundia M, Alemán-González-Duhart D, Correa-Basurto J, et
al. Study of new interactions of glitazone’s stereoisomers and the endogenous ligand 15d-PGJ2 on six different PPAR
gamma proteins. Biochemical Pharmacology. 2017;142:168-93. doi:10.1016/j.bcp.2017.07.012.
6. Hinds Jr TD, Adeosun SO, Alamodi AA, Stec DE. Does bilirubin prevent hepatic steatosis through activation of the PPARα
nuclear receptor? Medical hypotheses. 2016;95:54-7. doi:10.1016/j.mehy.2016.08.013.
7. Kumar S, Srivastava A, Palaia T, Hall C, Lee J, Stevenson M, et al. Lipocalin-type prostaglandin D2 synthase deletion
induces dyslipidemia and non-alcoholic fatty liver disease. Prostaglandins & other lipid mediators. 2020;149:106429.
doi:10.1016/j.prostaglandins.2020.106429.
8. Virtue S, Feldmann H, Christian M, Tan CY, Masoodi M, Dale M, et al. A new role for lipocalin prostaglandin d synthase
in the regulation of brown adipose tissue substrate utilization. Diabetes. 2012;61(12):3139-47. doi:10.2337/db12-0015.
9. Evans JF, Islam S, Urade Y, Eguchi N, Ragolia L. The lipocalin-type prostaglandin D2 synthase knockout mouse model
of insulin resistance and obesity demonstrates early hypothalamic-pituitary-adrenal axis hyperactivity. J Endocrinol.
2013;216(2):169-80. doi:10.1530/joe-12-0275.
10. Gaggini M, Morelli M, Buzzigoli E, DeFronzo RA, Bugianesi E, Gastaldelli A. Non-alcoholic fatty liver disease (NAFLD)
and its connection with insulin resistance, dyslipidemia, atherosclerosis and coronary heart disease. Nutrients.
2013;5(5):1544-60. doi:10.3390/nu5051544.
11. Kashef M, Salehpour M, Sadegh Ghomi M. Effect of eight weeks swimming exercise training on VEGF-A and FGF-2 of
hippocampus tissue and Lee's index in male wistar rats. Journal of Sport and Biomotor Sciences. 2019;22(22):10-8.
doi:10.22034/SBS.2019.161219. [In Persian].
12. Dehghan F, Cheragh Birjandi S. Effect of Obesity on Skeletal Muscle Semaphorin 3C Levels in Male and Female Wistar
Rats. Journal of North Khorasan University of Medical Sciences. 2022;3(14): 929. [In Persian].
13. Zoalfaghari F, Haghighi A, Hamedinia MR. The effect of circuit resistance exercise before exhausting running on treadmill
on fat, carbohydrate metabolism and energy expenditure in overweight and obese girls. Journal of Sabzevar University of
Medical Sciences. 2020;27(3):356-61. [In Persian].
14. Sepehri MH, Nemati J, Koushkie Jahromi M, Eskandari MH, Daryanoosh F. The effect of high-intensity interval training
on GLP-1, appetite, and weight in obese rats. Jundishapur Scientific Medical Journal. 2021;20(3):290-9.
doi:10.32598/JSMJ.20.3.1975. [In Persian].
15. Leandro CG, Levada AC, Hirabara SM, MANHAS-DE-CASTRO R, De-Castro CB, Curi R, et al. Aprogram of moderate
physical training for wistar rats based on maximal oxygen consumption. The Journal of Strength & Conditioning Research.
2007;3(21):751-6. doi:10.1519/r-20155.1.
16. Steki A, Valipour V, Ghahramanlo E, Kargarfard M. The effect of endurance training in air pollution on the expression of
brain cortex PGC-1α and Atf2 genes in Wistar male rats. KAUMS Journal (FEYZ). 2019;23(6):615-26.
doi:feyz.kaums.ac.ir/article-1-3955-fa.html. [In Persian].
42
17. Karbasi S, Zaeemi M ,Mohri M, Rashidlamir A, Moosavi Z. Effects of testosterone enanthate and resistance training on
myocardium in Wistar rats; clinical and anatomical pathology. Andrologia. 2018;50(3):e12908. doi:10.1111/and.12908.
18. Norozi M, Sadeghi A, Faal Pakdehi M, Torabi G. The effect of eight-week high intensity interval training (HIIT) and
caffeine consumption on glycogen synthase expression and hepatic glycogen levels in diabetic rats. Daneshvar Medicine.
2021;29(5):41-55. doi:10.22070/DANESHMED.2021.14819.1101. [In Persian].
19. Tanaka R, Miwa Y, Mou K, Tomikawa M, Eguchi N, Urade Y, et al. Knockout of the l-pgds gene aggravates obesity and
atherosclerosis in mice. Biochemical and biophysical research communications. 2009;378(4):851-6. doi:10.1016/j.bbrc.
2008.11.152.
20. Zhang L, Ma J, Jin X, Jia G, Jiang Y, Li C. L-PGDS mediates vagus nerve stimulation-induced neuroprotection in a rat
model of ischemic stroke by suppressing the apoptotic response. Neurochemical Research. 2017;42:644-55.
21. Ragolia L, Hall CE, Palaia T. Lipocalin-type prostaglandin D2 synthase stimulates glucose transport via enhanced GLUT4
translocation. Prostaglandins & other lipid mediators. 2008;87(1-4):34-41. doi:10.1016/j.prostaglandins.2008.06.001.
22. Srivastava A, Palaia T, Hall C, Stevenson M, Lee J, Ragolia L. Lipocalin-type prostaglandin D2 synthase appears to
function as a novel adipokine preventing adipose dysfunction in response to a high fat diet. Prostaglandins & Other Lipid
Mediators. 2021;157:106585. doi:10.1016/j.prostaglandins.2021.106585.
23. Elias E, Benrick A, Behre C, Ekman R, Zetterberg H, Stenlöf K, et al. Central Nervous System Lipocalin‐Type
Prostaglandin D2‐Synthase is Correlated with Orexigenic Neuropeptides, Visceral Adiposity and Markers of the
Hypothalamic‐Pituitary‐Adrenal Axis in Obese Humans. Journal of neuroendocrinology. 2011;23(6):501-7. doi:10.1111/
j.1365-2826.2011.02128.x.
24. Khairnar R, Islam MA, Kumar S. Investigating the Intertwine Effect of Insulin Resistance and Lipocalin Prostaglandin D2
Synthase in Non-alcoholic Fatty Liver Disease using HepG2 Cells. ASPET; 2023. doi:10.1124/jpet.122.539140.
25. Fujimori K, Aritake K, Oishi Y, Nagata N, Maehara T, Lazarus M, et al. L-PGDS-produced PGD2 in premature, but not
in mature, adipocytes increases obesity and insulin resistance. Scientific Reports. 2019;9(1):1931.
26. Ning H, Ren H, Zhao Y, Yin H, Gan Z, Shen Y, et al. Targeting the DP2 receptor alleviates muscle atrophy and diet‐
induced obesity in mice through oxidative myofiber transition. Journal of Cachexia, Sarcopenia and Muscle.
2023;14(1):342-55. doi:10.1002/jcsm.13136.
27. Wakai E, Aritake K, Urade Y, Fujimori K. Prostaglandin D2 enhances lipid accumulation through suppression of lipolysis
via DP2 (CRTH2) receptors in adipocytes. Biochemical and Biophysical Research Communications. 2017;490(2):393-9.
doi:10.1016/j.bbrc.2017.06.053.
28. Islam MA, Khairnar R, Fleishman J, Thompson K, Kumar S. Lipocalin-type prostaglandin D2 synthase protein-a central
player in metabolism. Pharmaceutical Research. 2022;39(11):2951-63.
29. Yang Y, Xiang P, Chen Q, Luo Y, Wang H, Li H, et al. The imbalance of PGD2-DPs pathway is involved in the type 2
diabetes brain injury by regulating autophagy. International Journal of Biological Sciences. 2021;17(14):3993. doi:10.
7150/ijbs.60149.
30. Civelek E, Ozen G. The biological actions of prostanoids in adipose tissue in physiological and pathophysiological
conditions. Prostaglandins, Leukotrienes and Essential Fatty Acids. 2022;186:102508. doi:10.1016/j.plefa.2022.102508.
31. Rasuli M, Zarrinkalam E. The Effect of Four Weeks of Aerobic Training on The Activity of Left Ventricular Catalase
Enzyme And Insulin Resistance Index In Male Rats Treated With Streptozotocin And Exposed To A High-Fat Diet.
Iranian Journal of Diabetes and Metabolism. 2022;22(4):255-64. doi:ijdld.tums.ac.ir/article-1-6175-fa.html. [In Persian].
32. Yaghoobpour Yekani O, Azarbayjani MA, Peeri M, Farzanegi P. The effect of aerobic training on anthropometric indices
of obesity in male rats fed with high fat diet. Medical Science Journal of Islamic Azad Univesity-Tehran Medical Branch.
2018;28(1):31-6. doi:10.29252/iau.28.1.31. [In Persian].
33. Daneshyar S, Ghasemnian A, Mirakhori Z, Daneshyar S. The effect of high fat diet and endurance training on newly
discovery of nonshivering-thermogenic factors under thermoneutrality in mice. Science & Sports. 2023;38(3):293-301.
doi:10.1016/j.scispo.2022.01.009. [In Persian].
34. Mancera-Soto EM, Chamorro-Acosta ML, Ramos-Caballero DM, Torrella JR, Cristancho-Mejía E. Effect of hypobaric
hypoxia on hematological parameters related to oxygen transport, blood volume and oxygen consumption in adolescent
endurance-training athletes. Journal of Exercise Science & Fitness. 2022;20(4):391-9. doi:10.1016/j.jesf.2022.10.003.
35. Said MA, Abdelmoneim MA, Alibrahim MS ,Kotb AAH. Aerobic training, resistance training, or their combination as a
means to fight against excess weight and metabolic syndrome in obese students—which is the most effective modality? A
randomized controlled trial. Applied Physiology, Nutrition ,and Metabolism. 2021;46(8):952-63.
36. Barzegari A, Asad MR, Dashti KhavIdaki MH, Eri E. The effect of high-Intensity Interval Training and Moderate-Intensity
Continuous Training on serum Irisin levels and insulin resistance in overweight men. Journal of Practical Studies of
Biosciences in Sport. 2023;11(27):34-46. doi:10.22077/jpsbs.2022.5305.1716. [In Persian].
37. Zhang H, Tong TK, Qiu W, Zhang X, Zhou S, Liu Y, et al. Comparable effects of high-intensity interval training and
prolonged continuous exercise training on abdominal visceral fat reduction in obese young women. Journal of diabetes
research. 2017;2017. doi:10.1155/2017/5071740

  • تاریخ دریافت 24 بهمن 1402
  • تاریخ بازنگری 05 تیر 1403
  • تاریخ پذیرش 07 تیر 1403