ورزش و علوم زیست حرکتی

ورزش و علوم زیست حرکتی

تأثیر تمرینات مقاومتی و مصرف مکمل اسپیرولینا بر مقادیر پلاسمایی اسپکسین، عوامل خطرزای قلبی-عروقی و برخی شاخص‌های ترکیب بدنی مردان دارای اضافه وزن

نوع مقاله : مقاله پژوهشی

نویسندگان
فهیمه ایمان پور 1
مهدی مقرنسی 2
شیلا نایبی فر 3
1 دانشجوی دکتری، گروه علوم ورزشی، دانشکده علوم ورزشی، دانشگاه بیرجند، بیرجند، ایران
2 استاد، گروه علوم ورزشی، دانشکده علوم ورزشی، دانشگاه بیرجند، بیرجند، ایران.
3 دانشیار، گروه علوم ورزشی، دانشکده علوم تربیتی و روانشناسی، دانشگاه سیستان و بلوچستان، زاهدان، ایران.
10.22034/sbs.2024.450252.1090
چکیده
مقدمه و هدف: در سال­‌های اخیر ورزش، رژیم ­غذایی و مکمل­ ها به عنوان راهی برای بهبود ترکیب بدنی، کاهش وزن و عوامل خطر مرتبط با چاقی شناخته شده‌اند. هدف از مطالعه حاضر بررسی تأثیر هشت هفته تمرین مقاومتی و مصرف مکمل اسپیرولینا بر مقادیر پلاسمایی اسپکسین، پروتئین واکنشگر-C ا(CRP) و برخی شاخص‌های ترکیب‌­بدنی مردان دارای اضافه وزن بود.
مواد و روش‌­ها: مطالعه حاضر نیمه‌تجربی و کاربردی بود. بدین منظور 44 مرد دارای اضافه­‌وزن (شاخص توده بدن >25 کیلوگرم بر مترمربع و میانگین وزن 5.48±93.83) کیلوگرم به طور تصادفی در چهار گروه تمرین + مکمل، تمرین + دارونما، مکمل و دارونما (هر گروه 11 نفر) قرار گرفتند. مداخلات به مدت هشت هفته و سه جلسه تمرین در هفته انجام شد. به این صورت که برنامه تمرین مقاومتی شامل تمرین با وزنه با الگوی زمان­بندی منعطف و در شدت­‌های مختلف (خیلی سبک، سبک، متوسط و سنگین) اجرا شد. در این مدت افراد گروه مکمل و دارونما، روزانه به ترتیب دو عدد کپسول 500 میلی­‌گرمی اسپیرولینا و دارونما مصرف کردند. داده­‌ها با استفاده از آزمون­‌های شاپیرو-ویلک، آنالیز کوواریانس، t زوجی و تعقیبی بونفرونی با سطح معنی­‌داری 0.05>P تجزیه و تحلیل شد.
یافته­‌ها: مقادیر اسپکسین، پروتئین واکنشگر-C، توده خالص‌بدنی (LBM) و مجموع آب بدن (TBW) آزمودنی‌­ها بین گروه‌­های تحقیق تفاوت معنی­‌داری را نشان دادند (0.05>P). همچنین در بررسی درون گروهی مقادیر پلاسمایی اسپکسین در گروه‌­های تمرین + مکمل، تمرین + دارونما  و مکمل، توده خالص بدنی (LBM)، در گروه تمرین + مکمل و تمرین + دارونما و مجموع آب بدن (TBW) در گروه تمرین + دارونما افزایش معنی‌­دار را نشان داد (0.05>P). در مقادیر پلاسمایی پروتئین واکنشگر-C نیز در گروه­‌های تمرین + مکمل، تمرین + دارونما و مکمل کاهش مشاهده شد (0.05>P).
بحث و نتیجه‌­گیری: به نظر می‌­رسد هشت هفته تمرین مقاومتی به همراه مصرف مکمل اسپیرولینا می‌­تواند از طریق افزایش مقادیر پلاسمایی اسپکسین، کاهش پروتئین واکنشگر-C و افزایش توده خالص بدن و مجموع آب بدن باعث بهبود ترکیب بدن و اختلالات مرتبط با چاقی نظیر بیماری‌­های قلبی- عروقی شود. بنابراین توصیه می­‌شود برنامه تمرین مقاومتی و مصرف مکمل اسپیرولینا برای پیشگیری و کاهش بیماری­‌های قلبی-عروقی و بهبود ترکیب‌­بدن مورد استفاده قرار گیرد.
واژه­‌های کلیدی: تمرین مقاومتی، اسپیرولینا، اسپکسین، پروتئین واکنشگر-C، توده‌­خالص بدن، مجموع آب بدن، اضافه­‌وزن و چاقی
کلیدواژه‌ها
تمرین مقاومتی
اسپیرولینا
اسپکسین
پروتئین واکنشگر-C
توده‌خالص بدن
مجموع آب بدن
اضافه‌وزن و چاقی

موضوعات

عنوان مقاله English

The effect of resistance training and spirulina supplementation on plasma levels of spexin, Cardiovascular risk factors and some body composition indices of overweight and obese men

نویسندگان English

Fahimeh Imanpoor 1
Mehdi Mogharnasi 2
Shila Nayebifar 3
1 PhD Candidate, Department of Sport Sciences, Faculty of Sport Sciences, University of Birjand, Birjand, Iran.
2 Professor, Department of Sport Sciences, Faculty of Sport Sciences, University of Birjand, Birjand, Iran.
3 Associate Professor, Department of Sport Sciences, Faculty of Education and Psychology, University of Sistan and Baluchestan, Zahedan, Iran.
چکیده English

Introduction and Purpose: In recent years, exercise, diet, and supplements have been known as ways to improve body composition, reduce weight, and mitigate risk factors related to obesity. The purpose of this study is to investigate the effect of eight weeks of resistance exercise and spirulina supplementation on plasma levels of spexin, C-reactive protein, and certain body composition indices in overweight and obese men.
Materials and Methods: The present study was semi-experimental and applied research. For this purpose, 44 overweight men (BMI > 25 kg/m², with an average weight of 83.93 ± 5.48 kg) were randomly assigned to four groups: training + supplement, training + placebo, supplement, and placebo (11 participants in each group). The interventions were conducted for eight weeks, with three sessions per week. The resistance training program included weight training with a flexible timing pattern and varying intensities (very light, light, medium, and heavy). During this period, participants in the supplement and placebo groups took two 500 mg capsules of spirulina or placebo daily, respectively. The data were analyzed using Shapiro-Wilk tests, analysis of covariance, paired t-tests, and Bonferroni post hoc tests, with a significance level of P<0.05.
Results: The plasma values of spexin in the supplement training group and the placebo training group were significantly higher than in the supplement and placebo groups (P<0.05). The plasma levels of C-reactive protein in the placebo group were significantly higher than in the other three groups. Lean body mass (LBM) in the supplement training group was significantly higher than in the supplement and placebo groups. Additionally, in the placebo training group, it was significantly higher than in the placebo group (P<0.05). Total body water (TBW) in the supplement training group was significantly higher than in the supplement and placebo groups, and it was also significantly higher in the placebo training group than in the supplement group (P<0.05).
Discussion and Conclusion: It appears that eight weeks of resistance training with spirulina supplementation can improve body composition and obesity-related disorders, such as cardiovascular diseases, by increasing plasma levels of spexin, decreasing plasma levels of C-reactive protein, and increasing LBM and TBW.

کلیدواژه‌ها English

Resistance training
Spirulina
Spexin
C-reactive protein
Lean body mass (LBM)
Total body water (TBW)
Overweight
Obesity
  1. Aktar N, Qureshi NK, Ferdous HS. Obesity: A review of pathogenesis and management strategies in adult.  Delta Medical College Journal. 2017; 5(1): 35 – 48. https://doi.org/10.3329/dmcj.v5i1.31436.
  2. Hwalla N, Jaafar Z. Dietary management of obesity: A review of the evidence. 2021; 11, 24. https://dx.doi.org/10.3390/diagnostics11010024.
  3. Koliaki C, Dalamaga M, Liatis S. Update on the obesity epidemic: after the sudden rise, is the upward trajectory beginning to flatten? Current Obesity Reports. 2023; 12:514-527. https://doi.org/10.1007/s13679-023-00527-y.
  4. Pone Simo L, Agbor VN, Temgoua FZ, Fosso Fozeu LC, Bonghaseh D.T, Mbonda AGN & et al. Prevalence and factors associated with overweight and obesity in selected health areas in a rural health district in Cameroon: a cross-sectional analysis. BMC Public Health. 2021; 21,475. https://dx.doi.org/10.1186/s12889-021-10403-w.
  5. Muller MJ, Geisler C. Defining obesity as a disease. European Journal of Clinical Nutrition. 2017; 71: 1256–1258. https://doi.org/10.1038/ejcn.2017.155
  6. Walewski JL, Ge F, Lobdell H, Levin N, Schwartz GJ, Vasselli J, & et al. Spexin is a novel human peptide that reduces adipocyte uptake of long chain fatty acids and causes weight loss in rodents with diet-induced obesity. Obesity. 2014; 22: 1643–1652. https://doi.org/10.1002/oby.20725.
  7. Amirpour M, Tarighat-Esfanjani A, Ghoreishi Z. Relationship between serum spexin levels and metabolic syndrome components in obese and normal weight people with or without diabetes. Medicine, Obesity. 2021; 24, 100337. https://doi.org/ 10.1016/j.obmed.2021.100337.
  8. Behrooz M, Vaghef-Mehrabany E, Ostadrahimi A. Different spexin level in obese vs normal weight children and its relationship with obesity related risk factors. Nutrition, metabolism, and cardiovascular diseases. 2019; 30, 4. https://doi.org/10.1016/j.numecd.2019.11.008.
  9. Kurowska P, Dawid M, Oprocha J, Respekta N, Serra L, Estienne A, & et al. Spexin role in human granulosa cells physiology and PCOS: expression and negative impact on steroidogenesis and proliferation. Biology of Reproduction. 2023; 109(5): 705-719. http://doi.org/10.1093/biolre/ioad108.
  10. Karaca A, Bakar-Ates F, Ersoz-Gulcelik N. Decreased spexin levels in patients with type 1 and type 2 diabetes. Medical Principle and Practice. 2018; 27:549–554. https://doi.org/10.1159/000493482.
  11. Mirabeau O, Perlas E, Severini C, Audero E, Gascuel O, Possenti R, & et al. Identification of novel peptide hormones in the human proteome by hidden Markov model screening. Genome Research. 2007; 17: 320–327. https://doi.org/10.1101/gr.5755407.
  12. Khadir A, Kavalakatt S, Madhu D, Devarajan S, Abubaker J, Al‑Mulla F, & et al. Spexin as an indicator of beneficial effects of exercise in human obesity and diabetes. Scientific RepoRts. 2020; 10(1):10635. https://doi.org/10.1038/s41598-020-67624-z.
  13. Lv S-Y, Zhou Y-C, Zhang X-M, Chen W-D, Wang Y-D. Emerging roles of NPQ/spexin in physiology and pathology. Pharmacol. 2019; 10, 457. https://doi.org/10.3389/fphar.2019.00457.
  14. Turkel I, Memi G, Yazgan B. Impact of spexin on metabolic diseases and inflmmation: An updated minireview. Experimental Biology and Medicine. 2022; 247:567-573. https://doi.org/10.1177/ 15353702211072443
  15. Gu L, Ma Y, Gu M, Zhang Y, Yan Sh, Li N, & et al. Spexin peptideis expressed in human endocrine and epithelial tissues and reduced after glucose load in type 2 diabetes. 2015; 71, 232–239. http://dx.doi.org/10.1016/j.peptides.2015.07.018
  16. Liu Y, Sun L, Zheng L, Su M, Liu H, Wei Y, & et al. Spexin protects cardiomyocytes from hypoxia-induced metabolic and mitochondrial dysfunction. Naunyn-Schmiedeberg's Archives of Pharmacology. 2020; 393(1): 25-33. https://doi.org/10.1007/s00210-019-01708-0.
  17. Kumar S, Hossain J, Javed A, Kullo I, Balagopal PB. Relationship of circulating spexin with markers of cardiovascular disease: A pilot study in adolescents with. Pediatric Obesity. 2018; 13(6): 374–380. https://doi.org/10.1111/ijpo.12249.
  18. Al-Daghri NM, Wani K, Yakout SM, Al-Hazmi H, Amer OE, Hussain SD, & et al. Favorable changes in fasting glucose in a 6-month self- monitored lifestyle modification programme inversely affects spexin levels in females with prediabetes. Scientific RepoRts. 2019; 9,9454. http://doi.org/10.1038/s41598-019-46006-0.
  19. Baghersalimi M, Fathi R, Kazemi S. The effect of aerobic training on lipid accumulation product, visceral adiposity, triglyceride-glucose and mcauley indices in early pubertal obese/overweight girls. sport physiology. 2020; 12(46): 95-116. http://doi.org/10.22089/spj.2019.6787.1849. [In Persian]
  20. Laswati H, Anderiana M, Subadi I, Yuanita I. The effects of physical exercises on C-reactive protein in patients with post ischemic stroke. Folia medica Indonesiana. 2016; 52(3): 180-184. http://doi.org/10.20473/v52i3.5449.
  21. Kasapis Ch, Thompson PD. The effects of physical activity on serum C-reactive protein and inflammatory markers. Journal of American College of Cardiology. 2005; 45(10): 1563-1569. http://doi.org/10.1016/jacc.2004.12.077.
  22. Atashak S, Piri, M.; Jafari A, Azarbayjani M A. Effects of 10 weeks resistance training and ginger consumption on C-reactive protein and some cardiovascular risk factors in obese men. Physiology and Pharmacology. 2010; 3(14): 314-323. Available from: sid.ir/paper/75190/fa. [In persian]
  23. Kouhi F, Moradi F, Absazadegan M. Effect of resistance training on serum interleukin-18 and C-reactive protein in obese men. Jounal of Gorgan University of Medical Sciences. 2014; 16 (1) :1-8. Available from: sid.ir/paper/79117/fa. [In persian]
  24. Hedayati M, Hosseini kakhk SA, Amiriparsa T, Haghighi AH, Askari R, Chamari M. The effect of resistance training on hs-CRP and cystatin C concentration in obese girls. Daneshvar Medicine. 2009;16(6): 9-18. Available from: https://sid.ir/paper/456522/fa. [In Persian]
  25. Mirseyyedi M, Attarzadeh Hosseini S, mir E, Hejazi K. Changes in C-reactive protein, interleukin-6 and lipid biomarkers in sedentary middle-aged men after resistance exercise. Journal of Sabzevar University of Medical Sciences. 2014; 21(2): 283-292. Available from: https://sid.ir/paper/82393/fa. [In Persian]
  26. Akbarpour M, Samari Z. The effect of aerobic training and spirulina supplementation on resistin and C-reactive protein in women with type 2 diabetes with overweight. Feyz. 2020; 24(5): 576-584. Available from: https://sid.ir/paper/401727/fa.584 [In Persian]
  27. Hooshmand moghadam B, Kordi MR, Attarzade Hosseini SR, Davaloo T. Aerobic exercises and supplement spirulina reduce inflammation in diabetic men. Pars journal of medical sciences. 2018; 16 (4) :10-18. http://doi.org/ 10.52547/JMJ.16.4.10. [In Persian]
  28. Nikseresht M, Agha-Alinejad H, Azarbayjani M, Ebrahim Kh. effects of nonlinear resistance and aerobic interval training on cytokines and insulin resistance in sedentary men who are obese. Journal of Strength and Conditioning Research. 2014; 28(9): 2560–2568. http://doi.org/10.1519/JSC.0000000000000441. [In Persian]
  29. Dehghani K, Mogharnasi M, Saghebjoo M, Sarir H, Malekaneh M. The effect of eight weeks of circuit resistance training and spirulina supplementation on plasma levels of irisin and some body composition in overweight and obese men. Armaghan-e-Danesh. 2020; 25(3): 332-345. http://doi.org/10.52547/ armaghanj. 25.3.332. [In Persian]
  30. Hakimi M, Sheikholeslami-Vatani D, Alimohamadi M. Comparing the effect of 8-week resistance training with concurrent (resistance- massage) on leptin serum, lipid profile and body composition in overweight young male. Sport Physiology. 2015; 7(25): 15-32. Available from: https://sid.ir/paper/79681/fa. [In persian]
  31. Golestani F, Mogharnasi M, Erfani‑Far M, Abtahi‑Eivari S H. The effects of spirulina under high‑intensity interval training on levels of nesfatin‑1, omentin‑1, and lipid profiles in overweight and obese females: A randomized, controlled, single‑blind trial. Journal of Research in Medical Sciences. 2021; 26(1). https://doi.org/10.4103/jrms.JRMS-1317-20.
  32. Pappas A, Tsiokanos A, Fatouros I G, Poulios A, Kouretas D, Goutzourelas N, & et al. The effects of spirulina supplementation on redox status and performance following a muscle damaging protocol. International journal of molecular sciences. 2021; 22, 3559. https://doi.org/10.3390/ ijms22073559.
  33. Akbarpour M, Mehrabe E. The effect of aerobic exercise and spirulina supplementation on some cardiovascular risk factors in overweight women with type 2 diabetes. Sport biosciences. 2020; 2(12): 207-222. https://dio.org/10.22059/.2020.276235.1330. [In persian]
  34. Eskandari M, Pournemati P, Hooshmand Moghadam B, Norouzi J. The interactive effect of aerobic exercise and supplementation of blue-algae (spirulina) on anthropometric indexes and cardiovascular risk factors in diabetic men. Sadra Medical Sciences Journal. 2020; 8(1): 51-62. https://doi.org/10.30476/smsj. 2020.83630.1068. [In persian]
  35. Ghaedi H, Farsi S, Taghipour Asrami A, Kaka R. The effect of nonlinear resistance training with supplementation of spirulina on serum leptin and ghrelin in obese women. Journal of Applied Health Studies in Sport Physiology. 2019; 6(1): 69-77. https://dx.doi.org/10.22049/jassp.2019.26662.1272. [In persian]
  36. Fleck SJ. Non-linear periodization for general fitness & athletes. Journal of Human Kinetics Special Issue. 2011; 29(1): 41-5. http://doi.org/10.2478/v10078-011-0057-2.
  37. Malekaneh M, Dehghani K, Mogharnasi M, Saghebjoo M, Sarir H, Nayebifar S. The combinatory effect of spirulina supplementation and resistance exercise on plasma contents of adipolin, apelin, ghrelin, and glucose in overweight and obese men. Mediators of Inflammation. Vol. 2022, Article ID 9539286, 9 pages https://doi.org/10.1155/2022/9539286.
  38. Mohammadi A, Bijeh N, Moazzami M, Khodaei K, Rahimi N. Effect of exercise training on spexin level, appetite, lipid accumulation product, visceral adiposity index, and body composition in adult with type 2 diabetes. Biological research for nursing. 2022; 24(2): 152-162. http://doi.org/10.1177/10998004211050596.
  39. Leciejewska N, Pruszy nska-Oszmałek E, Mielnik K, Głowacki M, Lehmann TP, Sassek M, & et al. Spexin promotesthe proliferation and differentiation of C2C12 cells In vitro—The effect of exercise on SPX and SPX receptor expression in skeletal muscle in vivo. Genes. 2022; 13, 81. https://doi.org/10/3390/
  40. Santiago LAM, Neto LGL, Pereira GB, Leite RD, Mostarda CT, Monzani JOB & et al. Effects of resistance training on immunoinflammatory response, TNF-Alpha gene expression, and body composition in elderly women. Hindawi. 2018. https://doi.org/10.1155/2018/1467025
  41. Tunc-Ata M, Turgut G, Mergen-Dalyanoglu M, Turgut S. Examination of levels pentraxin-3, interleukin-6, and C-reactive protein in rat model acute and chronic exercise. Journal of Exercise Rehabilitation. 2017; 13(3): 279-283. http://doi.org/10.12965/jer.1734920.490.
  42. Mogharnasi M, Bagheri M. The effect of 12 weeks of circuit resistance training on C-reactive protein and lipid profile in inactive women. Journal of Sport Biosciences. 2014; 6(2): 233-244. http://doi.org /10.22059/jsb.2014.50863. [In Persian]
  43. Ribeiro AS, Tomeleri CM, Souza MF, Pina F, Luiz C, Schoenfeld BJ & et al. Effect of resistance training on C-reactive protein, blood glucose and lipidprofile in older women with differing levels of RT experience. AGE. 2015; 37, 109. http://doi.org/1007/s11357-015-9849-y.
  44. Rose GL, Skinner TL, Mielke GI, Schaumberg MA. The effect of exercise intensity on chronic inflammation: A systematic review and meta-analysis. Journal of science and medicine in Sport. 2021 Apr; 24(4): 345-351. https://dio.org/10.1016/j.jsams.2020.10.004.
  45. Kalafati M, Jamurtas AZ, Nikolaidis MG, Paschalis V, Theodorou AA, Sakellariou GK, & et al. Ergogenic and antioxidant effects of spirulina supplementation in humans. Medicine and Science in Sports and Exercise. 2010. http://doi.org/ 10.1249/MSS.0b013e3181ac7a45.
  46. Peterson MD, Sen A, Gordon PM. Influence of resistance exercise on lean body mass in aging adults: A meta-analysis. Medicine and science in sports and exercise. 2011; 43(2): 249–258. https://doi.org/10.1249/ 0b013e3181eb6265.
  47. Nabucoa HCG, Tomeleri CM, Junior PS, Fernandes RR, Cavalcante EF, Santos L, & et al. Effect of whey protein supplementation combined with resistance training on cellular health in pre-conditioned older women: A randomized, double- blind, placebo-controlled trial. Archives of Gerontology and Geriatrics. 2019; 82: 232-237. https://dio.org/10.1016/j.archger.2019.03.007.
  48. Akbarzadeh S, Amir Sasan R, Vakili J. The effect of eight weeks of resistance training (one set and three sets) on maximal strength and net body mass of untrained women. 3rd. International Conference on Sports Sciences. 2016. Iran, Tehran. [In Persian]
  49. McBride J M, Blaak J B, Triplett-McBride T. Effect of resistance exercise volume and complexity on EMG, strength, and regional body composition. European journal of appllied physiology. 2003; l (90): 626–632. https://dio.org/ 10.1007/s00421-003-0930-3.

Dehghani K, Mogharnasi M, Saghebjoo M, Malekaneh M, Sarir H. Effect of spirulina platensis green-blue algae consumption, and circuit resistance training (CRT) on lipid profile in overweight and obese middle-aged men. Journal of Birjand University of Medical Sciences. 2021; 28(3): 248-259.https://dio.org/ 10.32592/JBirjandUnivMedSci.2021.28.3.93. [In Persian]

ورزش و علوم زیست حرکتی
دوره 16، شماره 32
اسفند 1403
صفحه 1-14

  • تاریخ دریافت 17 فروردین 1403
  • تاریخ بازنگری 23 اردیبهشت 1403
  • تاریخ پذیرش 13 خرداد 1403