ورزش و علوم زیست حرکتی

ورزش و علوم زیست حرکتی

تاثیر 8 هفته تمرینات تناوبی شنا با شدت بالا بر سطح فاکتور شبه هسته‌ای ۲ مشتق از اریتروئید ۲ (Nrf2) و سوپراکسیددیسموتاز (SOD) در موش های صحرایی نر چاق مبتلا به استئاتوهپاتیت غیرالکلی

نویسندگان
محمد هاشمی عزیزلی 1
فرهاد دریانوش 2
هما شیخانی شاهین 1
علیرضا جوهری 2
1 موسسه آموزش عالی زند شیراز
2 دانشگاه شیراز
10.22034/sbs.2021.161252
چکیده
مقدمه و هدف: با افزایش بی­ تحرکی در دنیای مدرن امروزی، بسیاری از بیماری­ های متابولیکی ناشی از عدم فعالیت بدنی گسترش یافته­ اند. از جمله این بیماری­ ها می­ توان به بیماری استئاتوهپاتیت غیرالکلی اشاره کرد که با افزایش استرس اکسیداتیو همراه است. هدف از این پژوهش، بررسی تاثیر هشت هفته تمرینات تناوبی شنا با شدت بالا بر سطح فاکتور شبه­ هسته‌ای ۲ مشتق از اریتروئید ۲ (Nrf2) و سوپراکسید­دیسموتاز (SOD) در موش­ های صحرایی نر چاق مبتلا به استئاتوهپاتیت غیرالکلی (NASH) بود.
مواد و روش ­ها: در ابتدا موش­ های صحرایی در سن 6 تا 8 هفته به­ طور تصادفی به دو گروه سالم (20= n) و بیمار (رژیم پرچرب (HFD)) (n=20) تقسیم شدند. رژیم پرچرب به مدت 8 هفته ادامه داشت تا موش­ه ای صحرایی مبتلا به NASH شوند. پس از القا بیماری، گروه بیمار به­ طور تصادفی به 2 گروه بیمار - بی­ تحرک (10=n)، بیمار - شنا (10=n)،  تقسیم شدند؛ هم­چنین گروه سالم نیز به دو گروه سالم - بی­ تحرک (10=n) و گروه سالم - شنا (10=n) تقسیم گردیدند. تمرین HIIT شنا، شامل 20 نوبت 30 ثانیه ­ای شنا با 30 ثانیه استراحت بین هر نوبت (سه روز در هفته به مدت هشت هفته) بود. در تمرین تناوبی، بار اعمال شده در هفته اول، وزنه­ ای به میزان 7 درصد وزن بدن هر موش صحرایی بود و هر هفته 1 درصد به آن اضافه شد. پروتئین Nrf2 از بافت کبد و از طریق تکنیک وسترن بلات و SOD از سرم خون اندازه ­گیری شدند. برای مشخص نمودن تفاوت میان گروه­ ها از آزمون آماری آنووای یکراهه و آزمون تعقیبی بونفرونی (0.05>P < span lang="FA">) استفاده شد.
یافته‌ها: نتایج آنالیز آماری نشان داد در متغیر SOD، گروه بیمار بی­تحرک نسبت به سالم بی­ تحرک و سالم - شنا کاهش معنادار (0.05>P < span lang="FA">) و گروه سالم - شنا نسبت به بیمار - شنا افزایش معنادار وجود داشت (0.05>P < span lang="FA">). در متغیر Nrf2 گروه سالم - بی­ تحرک نسبت به گروه ­های بیمار - بی­ تحرک و بیمار - شنا افزایش معنادار وجود داشت (0.05>P < span lang="FA">). هم­چنین در گروه بیمار - شنا نسبت به بیمار - بی­ تحرک تغییر معناداری وجود نداشت(0.05
)
بحث و نتیجه­ گیری: تمرینات تناوبی شنا با شدت بالا در بیماران مبتلا به NASH، می­تواند بر سیستم آنتی­ اکسیدانی تاثیر مثبت داشته باشد با این­ وجود در این زمینه انجام پژوهش­ های دیگر ضروری است.
کلیدواژه‌ها
فاکتور شبه هسته‌ای ۲ مشتق از اریتروئید ۲
سوپراکسید دیسموتاز
فعالیت ورزشی شنای تناوبی با شدت بالا
استئاتوهپاتیت غیرالکلی

عنوان مقاله English

The effect of 8 weeks of high-intensity swimming interval training on the factor level of Erythroid-Derived 2-nuclear Factor 2 (Nrf2) and Superoxide Dismutase (SOD) in obese male rats with Non-alcoholic Steatohepatitis

نویسندگان English

Mohammad Hashemi Azizli 1
Farhad Daryanoosh 2
Homa Sheikhani Shahin 1
Alireza Jowhari 2
چکیده English

Introduction and purpose: With the increase of inactivity in today's modern world, many metabolic diseases caused by lack of physical activity have spread. Among these diseases, we can mention non-alcoholic steatohepatitis, which is associated with increased oxidative stress. This study aimed to investigate the effect of 8 weeks of high-intensity swimming interval training on the factor level of Erythroid-Derived 2-Nuclear Factor 2 (Nrf2) and superoxide dismutase (SOD) in obese male rats with non-alcoholic Steatohepatitis (NASH).
Material and methods: At the beginning, rats aged 6 to 8 years were randomly divided into healthy (n=20) and sick (high fat diet (HFD)) (n=20) groups. The high-fat diet lasted eight weeks until the rats became infected with NASH. After induction of the disease, the patient group was divided into 2 groups: disease-immobile (n=10), disease-swimming (n=10) by chance; Also, the healthy group was divided into two groups: healthy-immobile (n=10) and healthy-swimming (n=10). The HIIT swimming exercise consisted of 20 30-second swimming sessions with a 30-second break between sessions (three days a week for eight weeks). In the interval training load applied in the first week, a weight of 7% of each rat's body weight added, and 1% added to it every week. Nrf2 protein measured from liver tissue through the western blotting technique and SOD from blood serum. One-way ANOVA and Bonferroni post hoc test (p<0.05) were used to determine the differences between the groups.
Results: The results of statistical analysis showed that in the SOD variable, there was a significant decrease (P<0.05) in the inactive disease group compared to the sedentary and sedentary healthy group and a significant increase in the healthy-swimming group compared to the patient-swimming group (P<0.05). There was a significant increase in the Nrf2 variable of the healthy-inactive group compared to the patient-inactive and disease-swimming groups (P<0.05). There was also a non-significant increase in the disease-swimming group compared to the patient-immobile
Discussion and Conclusion: high-intensity swimming interval training in patients with NASH can have a positive effect on the anti-oxidant system, but it is necessary to conduct other research.
Key words: Erythroid-Derived 2-Nuclear Factor 2, Superoxide Dismutase, High-intensity swimming interval training, Non-alcoholic Steatohepatitis

کلیدواژه‌ها English

Erythroid-Derived 2-Nuclear Factor 2 (Nrf2)
Superoxide dismutase (SOD)
high-intensity swimming interval training
Non-alcoholic Steatohepatitis (NASH)
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ورزش و علوم زیست حرکتی
دوره 13، شماره 26 - شماره پیاپی 26
پاییز و زمستان
اسفند 1400
صفحه 66-76

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