Journal of Sports and Biomotor Sciences

Journal of Sports and Biomotor Sciences

The effect of High-intensity interval training with Portulaca Oleracea Extract Supplementation on sirtuin 6 and insulin resistance in rats with non-alcoholic fatty liver disease

Document Type : Original Article

Authors
Mehri Sadat Fatemi 1
Sadegh Cheragh Birjandi 2
Ali Kheyrandish 3
1 M.Sc Candidate, Department of Exercise Physiology, Department of Physical Education and Sport Sciences, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran
2 Assistant Professor, Department of Exercise Physiology, Department of Physical Education and Sport Sciences, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran.
3 Assistant Professor, Department of Motor Behavior, Birjand University of Technology, Birjand, Iran
10.22034/sbs.2024.451340.1091
Abstract
Introduction and Purpose: Non-alcoholic fatty liver disease (NAFLD) results from excessive triglyceride accumulation in the liver. NAFLD leads to inflammation and oxidative stress in the body. It appears that oxidative stress disrupts Sirtuin 6 (SIRT6) expression and accelerates telomere attrition. Therefore, the present study aims to investigate the effects of eight weeks of high-intensity interval training (HIIT) and purslane supplementation on SIRT6 levels and insulin resistance in rats with NAFLD.
Materials and methods: Twenty-five male Wistar rats, six weeks old, weighing between 160 and 185 grams, were randomly selected. To induce NAFLD, the rats were fed a high-fat diet for 12 weeks. They were then divided into five groups: healthy control, NAFLD control, exercise, purslane supplement, and exercise + purslane supplement. Purslane supplementation was administered to the relevant groups at a dose of 400 mg/kg based on body weight. The HIIT protocol lasted for eight weeks, five days per week, with seven one-minute repetitions at 90% of maximal speed, interspersed with two-minute active recovery periods at 20% of maximal speed. SIRT6 levels were measured using the Western blot method. Data were analyzed using one-way ANOVA and Tukey’s post-hoc test at a significance level of P≤0.05.
Results: Insulin resistance was significantly higher in the NAFLD control group compared to the healthy control group. This indicator was significantly lower in the exercise, purslane supplement, and exercise + purslane supplement groups compared to the NAFLD control group. Additionally, SIRT6 protein levels were significantly lower in the NAFLD control group compared to the healthy control group. However, SIRT6 levels were significantly higher in the exercise, purslane supplement, and exercise + purslane supplement groups than in the NAFLD control group (P≤0.05).
Discussion and Conclusion: It appears that the combination of high-intensity interval training and purslane supplementation may serve as an adjunct therapy for individuals with non-alcoholic fatty liver disease.
Keywords
High-intensity interval training
Purslane supplement
Sirtuin 6
Insulin resistance
Non-alcoholic fatty liver disease

Subjects

  1. 1. Kazemi M, Abdi A, Barari A, Mehrabani J. The effect of interval training and omega-3 on endoplasmic reticulum stress in the liver tissue of nonalcoholic fatty liver disease (NAFLD) rats. Medical Science Journal of Islamic Azad Univesity-Tehran Medical Branch. 2024;34(1):25-36.[61186/iau.34.1.3] [In Persian]
  2. 2. Khaleghzadeh H, Afzalpour ME, Ahmadi MM, Nematy M, Sardar MA. Effect of high intensity interval training along with Oligopin supplementation on some inflammatory indices and liver enzymes in obese male Wistar rats with non-alcoholic fatty liver disease. Obesity Medicine. 2020;17:100177.[1016/ j.obmed.2019.100177]
  3. 3. Ranaei M, Yaghoubi A, Cheragh-Birjandi S. Effect of high-intensity interval training and portulaca oleracea extract supplementation on FGF-21 level and insulin resistance in rats with non-alcoholic fatty liver disease. Journal of Shahid Sadoughi University of Medical Sciences. 2023.[18502/ssu.v31i3.12806] [In Persian]
  4. 4. Bedalov A, Chowdhury S, Simon J. Biology, chemistry, and pharmacology of sirtuins. Methods in enzymology. 574: Elsevier; 2016. p. 183-211.[1016/bs.mie.2016.03.011]
  5. 5. Nassir F, Ibdah Sirtuins and nonalcoholic fatty liver disease. World journal of gastroenterology. 2016;22(46):10084.[10.3748/wjg.v22.i46.10084]
  6. 6. Keyvani F, Kordi MR, Maghbooli Taghidizaj Z, Shabkhiz F. The effect of eight weeks high intensity interval training on serum levels of telomerase enzyme and sirtuin 6 protein in aged men. Sport Physiology. 2020;12(45):17-30.[org/10.22089/spj.2019.1608] [In Persian]
  7. 7. Hou T, Tian Y, Cao Z, Zhang J, Feng T, Tao W, et al. Cytoplasmic SIRT6-mediated ACSL5 deacetylation impedes nonalcoholic fatty liver disease by facilitating hepatic fatty acid oxidation. Molecular Cell. 2022;82(21):4099-115. e9.[10.1016/j.molcel.2022.09.018]
  8. 8. Zhou S, Tang X, Chen H-Z. Sirtuins and insulin resistance. Frontiers in Endocrinology. 2018;9:421838 .[3389/ fendo.2018.00748]
  9. 9. Cheragh-Birjandi S, Kheyrandish A, Tara SS, Sharifian SS, Masoodi H, Asghari S. The effect of Pilates and aerobic training on serum levels of sirtoin-1, plasminogen activator inhibitor-1 and insulin resistance index in women with type 2 diabetes. Journal of Applied Health Studies in Sport Physiology. 2024(Articles in Press).[22049/jahssp.2024. 29241.1610] [In Persian]
  10. 10. Hosseini Z, Cheragh-Birjandi S. The effect of high-intensity interval training with portulaca oleracea hydroalcoholicextract on gene expression of selenoprotein P (SeP) and insulin resistance in rats with nonalcoholic fatty liver. Journal of Birjand University of Medical Sciences. 2023;30(3):257-67. [In Persian]
  11. 11. Ghorbanian B, Egtesadi S. The effect of high intensity interval training (HIIT) on serum levels of selenoprotein P and glycemic and body composition indices in women with nonalcoholic fatty liver disease. Journal of Applied Health Studies in Sport Physiology. 2022;9(1):12-21.[22049/jahssp.2021.27318.1367] [In Persian]
  12. 12. Winn NC, Liu Y, Rector RS, Parks EJ, Ibdah JA, Kanaley JA. Energy-matched moderate and high intensity exercise training improves nonalcoholic fatty liver disease risk independent of changes in body mass or abdominal adiposity—a randomized trial. Metabolism. 2018;78:128-40.[1016/j.metabol.2017.08.012]
  13. 13. Hallsworth K, Thoma C, Hollingsworth KG, Cassidy S, Anstee QM, Day CP, et al. Modified high-intensity interval training reduces liver fat and improves cardiac function in non-alcoholic fatty liver disease: a randomized controlled trial. Clinical science. 2015;129(12):1097-105.[1042/CS20150308]
  14. 14. Karvinen S, Silvennoinen M, Vainio P, Sistonen L, Koch LG, Britton SL, et al. Effects of intrinsic aerobic capacity, aging and voluntary running on skeletal muscle sirtuins and heat shock proteins. Experimental gerontology. 2016;79:46-54.[1016/j.exger.2016.03.015]
  15. 15. Kumar A, Sreedharan S, Kashyap AK, Singh P, Ramchiary N. A review on bioactive phytochemicals and ethnopharmacological potential of purslane (Portulaca oleracea L.). Heliyon. 2022;8(1).[10.1016/j.heliyon. e08669]
  16. 16. Changizi-Ashtiyani S, Zarei A, Taheri S, Rasekh F, Ramazani M. The effects of Portulaca oleracea alcoholic extract on induced hypercholesteroleomia in rats.Zahedan journal of research in medical sciences.2013;15 .(6)
  17. 17. Zarei M, Kadkhoda Z, Yaghoubi A. Effect of high-intensity interval training with portulaca oleracea supplementation on liver LXα and PPARγ receptors in rats with nonalcoholic fatty liver disease. Iranian Journal of Endocrinology and Metabolism. 2022;24.178.87:(3) [In Persian]
  18. 18. Ranaei M, Yaghoubi A, Cheragh-birjandi S. Changes in chemerin level and insulin resistance in rats with non-alcoholic fatty liver disease: effect of high intensity interval training and portulaca oleracea extract. medical journal of mashhad university of medical sciences. 2023;65(6):2273-84.[22038/mjms.2023.69700.4135] [In Persian]
  19. 19. Bedford TG, Tipton CM, Wilson NC, Oppliger RA, Gisolfi CV. Maximum oxygen consumption of rats and its changes with various experimental procedures. Journal of Applied Physiology. 1979;47(6):1278-83.[1152/jappl. 1979.47.6.1278]
  20. 20. Dehbashi M, Fathi M, Attarzadeh Hosseini SR, Mosaferi Ziaaldini M. The Effect of Eight Weeks Endurance Training, Somatropin Injection, and Its Lipolytic Fragment (AOD9604) on Cytokeratin-18 and Liver Enzymes of Mice Induced Liver Damage Due to a High-Fat Diet. Internal Medicine Today. 2021;27(4):502-17.[32598/hms.27.4.3513.1] [In Persian]
  21. 21. Zhang Y, Yang Z, Xu Z, Wan J, Hua T, Sun Q. Exercise ameliorates insulin resistance and improves SIRT6-mediated insulin signaling transduction in liver of obese rats. Canadian Journal of Physiology and Pharmacology. 2021;99(5):506-11.[1139/cjpp-2020-0083]
  22. 22. Kuang J, Chen L, Tang Q, Zhang J, Li Y, He J. The role of Sirt6 in obesity and diabetes. Frontiers in physiology. 2018;9:135.[3389/fphys.2018.00135/full]
  23. 23. Kanwal A, Pillai VB, Samant S, Gupta M, Gupta MP. The nuclear and mitochondrial sirtuins, Sirt6 and Sirt3, regulate each other’s activity and protect the heart from developing obesity-mediated diabetic cardiomyopathy. The FASEB Journal. 2019;33(10):10872.[1096/fj.201900767R]
  24. 24. Shahhosseini S, Ravasi A, Nouri R. Sirtuin 6 gene expression and telomere length ghanges in old rat hepatocytes after 6 weeks swimming exercise and resveratrol supplementation feeding. Studies in Medical Sciences. 2021;32(8):619-30.[52547/umj.32.8.619] [In Persian]
  25. 25. Mohammadkhani R, Khaledi N, Rajabi H, Komaki A, Salehi I. The influence of maternal high intensity interval training before and during pregnancy on the heart genes of adult female wistar offspring. Razi J Med Sci. 2023;29(10):258-267. [In Persian]
  26. 26. Lalei M, Haghshenas R. The effect of nordic training on plasma levels of peroxisome proliferator-activated receptor-γ coactivator 1-α and Sirtuin 6 in elderly women with diabetes. Koomesh. 2024;25(1):65-70.
    [In Persian]
  27. 27. Kazemi M, Abdi A, Mehrabani J, Barari A. The effect of interval training and omega-3 on mitochondrial biogenesis in the liver tissue of nonalcoholic fatty liver disease (NAFLD) rats. Armaghane Danesh. 2023;28(5):638-54.[61186/armaghanj.28.5.4] [In Persian]
  28. 28. Akbari A, Mohebbi H, Tabari E. The effects of high fat diet-induced obesity and interval and continuous exercise training on visceral fat SIRT1 and insulin resistance in male rats. Iranian Journal of Diabetes and Metabolism. 2020;19(2):93-102. [In Persian]
  29. 29. Afzalpour ME, Sarir H, Zanjirian Z, Moammadnia Ahmadi M, Ghasemi E. The effect of vigorous continuous and interval exercise training along with resveratrol on SIRT3 and OGG1 proteins in the liver tissue of male Wistar rats. Journal of Sport and Exercise Physiology. 2020;13(1):111-27.[52547/joeppa.13.1.111]
    [In Persian]
  30. 30. Koltai E, Szabo Z, Atalay M, Boldogh I, Naito H, Goto S, et al. Exercise alters SIRT1, SIRT6, NAD and NAMPT levels in skeletal muscle of aged rats. Mechanisms of ageing and development. 2010;131(1):21-8.[1016/j.mad.2009.11.002]
  31. 31. Taran S, Ghorbanian B, Tutunchi H. The effect of eight weeks of Pilates exercise on serum levels of fibroblast growth factor 21 (FGF21), insulin resistance index and lipid profile in women with non-alcoholic fatty liver disease. Journal of Applied Health Studies in Sport Physiology. 2024.[22049/jahssp.2024.29350.1622]
    [In Persian]
  32. 32. Khorasani-Toroghi T, Yaghoubi A. Effect of high intensity interval training and portulaca oleracea supplement on changes in fetuin a level and insulin resistance in rats with non-alcoholic fatty liver disease. Journal of Mazandaran University of Medical Sciences. 2022;32(212):29-41. [In Persian]
  33. 33. Stepto N, Hiam D, Gibson-Helm M, Cassar S, Harrison C, Hutchison S, et al. Exercise and insulin resistance in PCOS: muscle insulin signalling and fibrosis. Endocrine connections. 2020;9(4):346-59.[1530/EC-19-0551]
Journal of Sports and Biomotor Sciences
Volume 16, Issue 32
March 2025
Pages 61-70

  • Receive Date 06 April 2024
  • Revise Date 06 September 2024
  • Accept Date 06 September 2024