Journal of Sports and Biomotor Sciences

Journal of Sports and Biomotor Sciences

The effect of aerobic training on the mir-195 gene expression in heart tissue of type 2 diabetic rats

Document Type : Original Article

Authors
Elaheh Piralaiy 1
Badrkhan Rashwan Ismael 2
Gholamreza Hamidian 3
1 Assistant Professor, Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, Tabriz University, Tabriz, Iran.
2 Ph.D Candidate, Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, Tabriz University, Tabriz, Iran.
3 3. Associate Professor in Comparative Histology, Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
10.22034/sbs.2024.460274.1101
Abstract
Introduction and Purpose: In recent years, a strong association has been identified between miRNA and diseases such as diabetes and cardiovascular disorders. Therefore, this study aimed to investigate the effect of eight weeks of aerobic exercise on the expression of the mir-195 gene in the heart tissue of rats with type 2 diabetes.
Materials and methods: For this purpose, 18 male Wistar rats (average weight: 240 g, average age: 8 weeks) were randomly assigned to three groups: (1) healthy control, (2) diabetic control, and (3) diabetic exercise. To induce type 2 diabetes, the rats were first fed a high-fat diet (60%) for two weeks, followed by fasting streptozotocin injection. The treadmill running protocol was performed five days a week, following the principle of progressive overload. In the first week, the exercise was conducted at a speed of 5–10 m/min for 10–15 minutes, and by the eighth week, it was increased to 18–24 m/min for 60 minutes. Gene expression of miR-195 was assessed using real-time PCR. One-way ANOVA and Tukey’s post hoc test were used for statistical analysis at a significance level of P≤0.05.
Results: Eight weeks of T2DM led to a significant increase in glucose levels and miR-195 gene expression (P≤0.05). However, eight weeks of aerobic exercise significantly reduced glucose levels and miR-195 gene expression compared to the diabetic control group (P≤0.05).
Discussion and Conclusion: Our results suggest that exercise, particularly aerobic training, is an effective strategy for reducing miR-195 expression and improving myocardial function in diabetic rats.
Keywords
Aerobic training
mir-195 gene
Heart tissue
Rats
Type 2 diabetes

Subjects

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Journal of Sports and Biomotor Sciences
Volume 16, Issue 32
March 2025
Pages 39-49

  • Receive Date 01 June 2024
  • Revise Date 14 September 2024
  • Accept Date 28 September 2024