Journal of Sports and Biomotor Sciences

Journal of Sports and Biomotor Sciences

The effect of weekly combined exercise (aerobics and pilates) on the serum level of antioxidant biomarkers in women with breast cancer

Document Type : Original Article

Authors
Bahloul Ghorbanian 1
yousef saberi 2
elnaz hamidi 3
1 Associate Professor, Department of Sport Sciences, Faculty of Psychology and Education, Azarbaijan Shahid Madani University, Tabriz, Iran.
2 Ph.D, Department of Sport Sciences, Faculty of Psychology and Education, Azarbaijan Shahid Madani University, Tabriz, Iran
3 M.Sc, Department of Sport Sciences, Faculty of Psychology and Education, Azarbaijan Shahid Madani University, Tabriz, Iran.
10.22034/sbs.2024.445065.1084
Abstract
Introduction and Purpose: Studies show that there is a relationship between the levels of endogenous antioxidants and the risk of breast cancer and its recurrence. Sports activity is recognized as an effective way to prevent and treat cancer. On the other hand, the role of combined sports activities, especially Pilates and aerobics, in influencing these factors in cancer patients is not clear. The aim of the present study was to investigate changes in certain endogenous antioxidants in postmenopausal women with breast cancer after 12 weeks of combined sports activity.
Materials and Methods: In this semi-experimental study, 30 women with breast cancer undergoing treatment were randomly selected as available subjects and divided into two groups: experimental and control. The experimental group participated in Pilates and aerobics for 12 weeks, with three sessions per week, while the control group did not engage in any sports program during this period. Before and after the training intervention, blood samples were taken to assess the studied variables. The data were analyzed using independent and dependent t-tests at a significance level of less than 0.05.
Results: The results showed that after 12 weeks of combined training, the levels of glutathione peroxidase (P=0.005) and total antioxidant capacity in the experimental group significantly increased compared to the control group (P<0.05). However, changes in the levels of superoxide dismutase and malondialdehyde were not significant (P>0.05).
Conclusion: It appears that combined aerobic and Pilates training can have beneficial effects on enzymatic antioxidant defense by increasing GPX, SOD, and TAC levels in women with breast cancer, which may help reduce the recurrence or metastasis of breast cancer
Keywords
Combined exercise
Breast cancer
Superoxide dismutase
Glutathione peroxidase
Malondialdehyde
Total antioxidant capacity

Subjects

  1. Rajendra C, Kedari G. Role of antioxidant levelsand lipid peroxidationin patients with breast cancer. Biomedicine. 2020;40:170-174 . doi.org/10.1016/j.phrs.2023.106777.
  2. Kangari P, Farahany T. Z, Golchin A, Ebadollahzadeh S, Salmaninejad A, Mahboob S. A, et al . Enzymatic antioxidant and lipid peroxidation evaluation in the newly diagnosed breast cancer patients in Iran. Asian Pacific journal of cancer prevention. 2019; 19: 351. doi: 10.31557/APJCP.2018.19.12.3511.
  3. Youssef H, Salem H. Oxidative stress parameters in patients with breast cancer before and after radiotherapy. Egyptian Journal of Radiation Sciences and Applications.2019;32:177-185. doi:  10.21608 /EJRSA.2019. 15164.1079.
  4. Shamshirian A, Heydari K, Shams Z, Aref A. R, Shamshirian D, Tamtaji O. R, et al. Breast cancer risk factors in Iran: a systematic review & meta-analysis. Hormone molecular biology and clinical investigation.2020; 41: 21. doi: 10.1515/hmbci-2020-0021.
  5. Harris I. S, DeNicola G. M, The complex interplay between antioxidants and ROS in cancer. Trends in cell biology.2002; 30: 40-451. doi: 10.1016/j.tcb.2020.03.002.
  6. Cui Q, Wang J. Q, Assaraf Y. G, Ren L, Gupta P, Wei L, et al. Modulating ROS to overcome multidrug resistance in cancer. Drug Resistance Updates. 2018; 41: 1-25. doi: 10.1016/j.drup.2018.11.001.
  7. Brenneisen P, Reichert A. S. Nanotherapy and reactive oxygen species (ROS) in cancer: a novel perspective. Antioxidants.2018; 7: 31.. doi: 10.3390/antiox7020031.
  8. Khan A. Q, Rashid K, AlAmodi A, Agha M.V, Akhtar S, Hakeem I, et al. Reactive oxygen species (ROS) in cancer pathogenesis and therapy: An update on the role of ROS in anticancer action of benzophenanthridine alkaloids. Biomedicine & Pharmacotherapy.2021; 143: 112-142. doi: 10.1016/j.biopha.2021.112142.
  9. Martínez-Reyes I, Chandel N. S. Cancer metabolism: looking forward. Nature Reviews Cancer.2021; 21: 669-680. doi: 10.1038/s41568-021-00378-6 .
  10. Niu B, Liao K, Zhou Y, Wen T, Quan G, Pan X, et al. Application of glutathione depletion in cancer therapy: Enhanced ROS-based therapy, ferroptosis, and chemotherapy. Biomaterials. 2021; 277: 110-121. 10.1016/j.biomaterials. 2021.121110.
  11. Hernansanz-Agustín P, Enríquez J. Generation of reactive oxygen species by mitochondria. Antioxidants.2021;10: 415. doi: 10.3390/antiox10030415.
  12. Encarnación-Rosado J, Kimmelman A. C. Harnessing metabolic dependencies in pancreatic cancers. Nature Reviews Gastroenterology & Hepatology.2021; 18: 482-492. doi:  10.1038/s41575-021-00431-7.
  13. Buico A, Cassino C, Ravera M, Betta P.G, Osella D. Oxidative stress and total antioxidant capacity in human plasma. Redox Report. 2009; 14: 125-131. doi: 10.1179/135100009X392557.
  14. Cecchini S, Fazio F. Assessment of total antioxidant capacity in serum of heathy and stressed hens. Animals. 2020;10(11):2019. doi:10.3390/ani10112019.
  15. Omar ME A.S, Eman R.Y, Hafez H.F. The antioxidant status of the plasma in patients with breast cancer undergoing chemotherapy. Open Journal of Molecular and Integrative Physiology. 2011;1(3):29-35. doi: 10.4236/ojmip.2011.13005.
  16. Tas F, Hansel H, Belce A, Ilvan S, Argon A, Camlica H, Topuz E. Oxidative stress in breast cancer. Medical Oncology. 2005;22(1):11-5.  doi: 10.1385/MO:22:1:011.
  17. Didziapetriene J, Smailyte G, Bublevic J, Kazbariene B, Kasiulevicius V, Stukas R. Relationship of MDA plasma concentrations to long-term survival of breast cancer patients. Tumori Journal. 2014; 100(3):333-7. doi:10.1700/1578.17220.
  18. Qi Y, Min H, Mujeeb A, Zhang Y, Han X, Zhao X, et al. Injectable hexapeptide hydrogel for localized chemotherapy prevents breast cancer recurrence. ACS applied materials & interfaces. 2018;10(8):6972-6981.  doi: 10.1021/acsami.7b19258.
  19. Shahar S, Salleh R. M, Ghazali A. R, Koon P. B, Mohamud W. Roles of adiposity, lifetime physical activity and serum adiponectin in occurrence of breast cancer among Malaysian women in Klang Valley. Asian Pacific Journal of Cancer Prevention. 2010;11(1):61-6. PMID: 20593932.
  20. Irwin M. L, Smith A. W, McTiernan A, Ballard-Barbash R, Cronin K, Gilliland F. D, et al. Influence of pre-and postdiagnosis physical activity on mortality in breast cancer survivors: the health, eating, activity, and lifestyle study. Journal of clinical oncology. 2008 Aug 20; 26(24): 3958–3964 .doi: 10.1200/JCO .2007.15.9822.
  21. Karimi F. Behboudi Tabrizi L. The Effects of Six Weeks of Aerobic Training on the Tumor Volume and Oncostatin-M Serum Level in Mice with Breast Cancer. Journal of Rafsanjan University of Medical Sciences. 2018; 17(2): 105-14. URL: http://journal.rums.ac.ir/article-1-3839-en.html [in persian]
  22. Suner-Keklik S, Numanoglu-Akbas A, Cobanoglu G, Kafa N, Guzel NA. An online pilates exercise program is effective on proprioception and core muscle endurance in a randomized controlled trial. IrishJournal of Medical Sciences. 2022;191(5):2133-2139. doi: 10.1007/s11845-021-02840-8.
  23. Fernández-Rodríguez R, Álvarez-Bueno C, Cavero-Redondo I, Torres-Costoso A, Pozuelo-Carrascosa DP, Reina-Gutiérrez S, et al. Best Exercise Options for Reducing Pain and Disability in Adults With Chronic Low Back Pain: Pilates, Strength, Core-Based, and Mind-Body. A Network Meta-analysis. Journal of Orthopaedic & Sports Physical Therapy. 2022; 52(8):505-521. doi: 10.2519/jospt .2022. 10671.
  24. Gou Y, Lei H, Zeng Y, Tao J, Kong W, Wu J. The effect of Pilates exercise training for scoliosis on improving spinal deformity and quality of life: Meta-analysis of randomized controlled trials. Medicine (Baltimore). 2021;100(39):e27254. doi: 10.1097/MD.0000000000027254.
  25. Wang Y, Chen Z, Wu Z, Ye X, Xu X. Pilates for overweight or obesity: a meta-analysis. Frontiers in physiology. 2021 Mar 11;12:643455. doi: 10.3389/fphys.2021.643455.
  26. Ferguson B. ACSM’s Guidelines for Exercise Testing and Prescription 9th Ed. 2014. J Can Chiropr Assoc. 2014;58(3):328. PMCID: PMC4139760.
  27. Yadav, Alok Kumar; Bagi, Jayasheela G. A study to evaluate cardiovascular responses by using treadmill and ergometer bicycle exercise in young adults. Indian Journal of Health Sciences and Biomedical Research (KLEU). 2018; 11(1):p 81-85. doi: 10.4103/kleuhsj.kleuhsj 8917
  28. 28. Courneya KS, Segal RJ, Mackey JR, Gelmon K, Reid RD, Friedenreich CM, et al. Effects of aerobic and resistance exercise in breast cancer patients receiving adjuvant chemotherapy: a multicenter randomized controlled trial. Journal of Clinical Oncology. 2007;25(28):4396-404. doi: 10.1200/JCO. 2006.08.2024.

29 .Azamian Jazi A, Emd S, Hemati S. Effect of six weeks of continuous running on oxidative stress, lipid peroxidation and aerobic power in female survivors of breast cancer. Iranian Journal of Obstetrics, Gynecology and Infertility. 2017; 19(38): 24-32. doi: 10.22038/ijogi.2017.8278.

  1. 30. Karimi N, Roshan VD. Change in adiponectin and oxidative stress after modifiable lifestyle interventions in breast cancer cases.  Asian Pacific Journal of Cancer Prevention. 2013;14(5):2845-50. doi: 10.7314/apjcp.2013.14.5.2845.

31 .Repka CP, Hayward R. Oxidative stress and fitness changes in cancer patients after exercise training. Medicine & Science in Sports & Exercise. 2016 Apr;48(4):607-14. doi:10.1249/MSS.0000000000000 821.

32 .Emadi S, Azamian Jazi A. Hemati S. Effect of 6 Weeks of Low-volume High-intensity Interval Training on Antioxidant Defense and Aerobic Power in Female Survivors of Breast Cancer 2018; 60(6): 779-791. doi: 10.22038/mjms.2018. 10742 [in persian]

  1. 33. Pandey KB, Rizvi SI. Biomarkers of oxidative stress in red blood cells. Olomouc Czech Republic. 2011;155(2):131-6. doi: 10.5507/bp.2011.027. PMID: 21804621.
  2. 34. Park SY, Kwak YS. Impact of aerobic and anaerobic exercise training on oxidative stress and antioxidant defense in athletes. Journal of Exercise Rehabilitation. 2016;12(2):113-7. doi: 10.12965 /jer.

35 .Taghiyar S, Shahanipoor K, Razmi N A. Comparison of the Serum Level of Carcinoembryonic antigen, Prolactin, and Malondialdehyde in Patients Suffering from Breast Cancer and in Healthy individuals in Isfahan. Journal of Advanced Biomedical Sciences. 2014; 4 (3) :362-370. URL: http://jabs.fums.ac.ir/article-1-180-en.html.

  1. 36. Mazdak H, Mirkheshti N, Movahedian A, Yazdekhasti F, Behzad E, Shafieian M. Study of the oxidative stress markers in bladder cancer patients in comparison with healthy subjects. Razi Journal of Medical Sciences. 2009; 16 :167-172. URL: http://rjms.iums.ac.ir/article-1-1180-en.html.
  2. 37. Negahdar M, Jalali M, Abtahi H, Sadeghi M, Javadi E, Aghvami T, et al. Blood superoxide dismutase and catalase activities in women affected with breast cancer . Iran Journal Public Health. 2005; 1;34(3):39-43.
  3. 38. Rajneesh CP, Manimaran A, Sasikala KR, Adaikappan P. Lipid peroxidation and antioxidant status in patients with breast cancer. singapore medical journal. 2008;49(8):640-3. PMID: 18756349.
  4. 39. Miyazaki H, Oh-ishi S, Ookawara T, Kizaki T, Toshinai K, Ha S, et al. Strenuous endurance training in humans reduces oxidative stress following exhausting exercise. European Journal of Applied Physiology. 2001;84(1-2):1-6. doi: 10.1007/s004210000342.
  5. 40. Hatao H, Oh-ishi S, Itoh M, Leeuwenburgh C, Ohno H, Ookawara T, et al. Effects of acute exercise on lung antioxidant enzymes in young and old rats. Mechanisms of Ageing and Development. 2006; 4: 384-390. https://doi.org/10.1016/j.mad.2005.12.008 

41 .Goon JA, Aini AH, Musalmah M, Anum MY, Nazaimoon WM, Ngah WZ. Effect of Tai Chi exercise on DNA damage, antioxidant enzymes, and oxidative stress in middle-age adults.  Journal of Physical Activity and Health. 2009; 6(1):43-54. doi: 10.1123/jpah.6.1.43.

42 .Marsh SA, Laursen PB, Coombes JS. Effects of antioxidant supplementation and exercise training on erythrocyte antioxidant enzymes. International Journal for Vitamin and Nutrition Research. 2006; 76(5):324-31. doi: 10.1024/0300-9831.76.5.324.

  1. 43. Nori R, |Mahmoudyeh B, Rahmaninia F, Damirchi A. Changes in the levels of endogenous antioxidants in postmenopausal women with breast cancer after 15 weeks of combined sports activity; A randomized clinical trial. Journal of Applied Exercise Physiology (JAEP) 2016; 11(22): 53-6 , doi: 10.22080/jaep.2016.1208. [In Persian].
  2. 44. Souza-Rabbo MP, Silva LF, Auzani JA, Picoral M, Khaper N, Belló-Klein A. Effects of a chronic exercise training protocol on oxidative stress and right ventricular hypertrophy in monocrotaline‐treated rats. Clinical and Experimental Pharmacology and Physiology. 2008; 35(8):944-8. doi: 10.1111/j.1440-1681.2008.04936.x. Epub 2008 Apr 21.

45 .Lambertucci RH, Levada-Pires AC, Rossoni LV, Curi R, Pithon-Curi TC. Pithon-Curi. Effects of aerobic exercise training on antioxidant enzyme activities and mRNA levels in soleus muscle from young and aged rats. Mechanisms of Ageing and Development. 2007;128(3):267-75. doi: 10.1016/j .mad.2006.12.006. Epub 2006 Dec 23.

  1. 46. Robertson JD, Maughan RJ, Duthie GG, Morrice PC. Increased blood antioxidant systems of runners in response to training load. Clinical science (Lond). 1991;80(6):611-8. doi: 10.1042/cs0800611.
  2. 47. Chevion S, Moran DS, Heled Y, Shani Y, Regev G, Abbou B, et al. Plasma antioxidant status and cell injury after severe physical exercise. Proceedings of the National Academy of Sciences U S A. 2003;100(9):5119-23. doi: 10.1073/pnas.0831097100.
  3. 48. Jyothsna K, Kadirvelu J. Oxidative stress and antioxidant status in rat blood, liver and muscle: effect of dietary lipid, carnitine and exercise. International journal for vitamin and nutrition research. 2005; 75(5): 333–339. org/10.1024/0300-9831.75.5.333
  4. 49. Paine SK, Basu A, Mondal LK, Sen A, Choudhuri S, Chowdhury IH, et al. Association of vascular endothelial growth factor, transforming growth factor beta, and interferon gamma gene polymorphisms with proliferative diabetic retinopathy in patients with type 2 diabetes. Molecular Vision. 2012;18:2749-57. PMID: 23213275.
  5. 50. Aslan R, Mehmet R, Tarakcioglu Effect of acute and regular exercise onantioxidative enzymes, tissue damage markersand membran lipid peroxidation of erythrocytesin sedentary students. Turkish Journal of Medical Sciences. 1998;28: 411-414 .
  6. 51. Tupurani M. A, Padala C, Kumar R. G, Puranam K, Kumari S, Rani S. H. Oxidative stress/Nitrosative stress in breast cancer. International Journal of Analytical Bio-Science. 2020;13(1):46-54.
  7. 52. Thirupathi A, Wang M, Lin JK, Fekete G, István B, Baker JS, Gu Y. Effect of different exercise modalities on oxidative stress: a systematic review. BioMedical Research International. 2021; 1947 92 . doi: 10.1155/2021/1947928.

 

Journal of Sports and Biomotor Sciences
Volume 16, Issue 32
March 2025
Pages 15-27

  • Receive Date 21 February 2024
  • Revise Date 19 October 2024
  • Accept Date 29 October 2024