Journal of Sports and Biomotor Sciences

Journal of Sports and Biomotor Sciences

The effect of reducing physical activity after a period of endurance training on the expression of STIM1, Orai1 and MG29 genes in the plantaris muscle of male Wistar rats

Document Type : Original Article

Authors
Abdolreza Kazemi 1
Mokhtar Ghanbarzadeh 2
Ziya Navidi 3
Shima Emam Bakhsh 4
1 Associated Professor, Department of Sports Sciences, School of literature and Humanities, Vali-E-Asr University, Rafsanjan, Iran
2 MSc, Department of Sports Sciences, School of literature and Humanities, Islamic Azad University of Kerman, Kerman, Iran
3 Associated Professor, Department of anesthesiology, School of medicine, rafsanjan university of medical sciences, rafsanjan, iran
4 4. MSc, Department of Sports Sciences, School of literature and Humanities, Islamic Azad University of Kerman, Kerman, Iran
10.22034/sbs.2022.162735
Abstract
Introduction and purpose: Decreased physical activity in rodents has caused a decrease in muscle mass, cross-sectional area of myofibrils (CSA) and force production. The purpose of this study was to investigate the effect of reducing physical activity after a period of endurance training on the expression of STIM1, Orai1 and MG29 genes in the plantaris muscle of male Wistar rats.
Materials and methods: The current research is experimental and basic. 16 male white desert rats, Wistar breed, weighing 250 ± 20 grams, were randomly divided into two groups. Control group - Spinal nerve ligation (SNL): This group included 8-week-old male rats whose three sciatic branches were tightly tied. The second group included 8 rats who received SNL after a period of endurance training. They did the endurance training protocol in the present study was for 4 days a week for 6 weeks. It increased from 10 meters per minute for 10 minutes in the first week to 17-18 meters per minute for 30 minutes in the fifth week. After the last training session, rats were anesthetized by intraperitoneal injection of ketamine and xylazine, and muscle tissue was extracted. To determine the significance of the difference between the variables, independent t-test was used, and SPSS-22 software was used to analyze the data with a significance level of P>0.05
Results: The results of the present research showed that endurance training before SNL caused a significant change in the MG29 gene (P=0.07) and a significant increase in the Stim1 gene and Orai1 (P>0.05).
Discussion and Conclusion: Endurance training was able to modulate the expression disorder of these proteins in the state of neuropathy, which may be due to various mechanisms such as controlling oxidative stress factors and growth factors, in this way exerts its beneficial effects.
Keywords
Decreased activity
STIM1
Orai1
MG29
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Journal of Sports and Biomotor Sciences
Volume 14, Issue 27 - Serial Number 27
September 2022
Pages 92-101

  • Receive Date 08 August 2022
  • Accept Date 06 November 2022