Introduction: High intensity exercise training increases MCTs, NHEs and NBCs gene expression but the effect of endurance training on NHEs and NBCs gene and protein expression is uncertain. Therefore, the purpose of this study was to investigate the effect of endurance training on myocardial NHE1 and NBC1 mRNA expressions in rat. 
Methods: 20 male Wistar rats (4 weeks age and 93.7±9.8g in weight) were selected and randomly divided into control and training groups. The rats were exposed to endurance training for 7 weeks (20m∕min and 20 min respectively) in the first week and reached to 30m∕min and 35min gradually in the last week. NHE1 and NBC1 mRNA expressions were determined by Real time-PCR technique in myocardial preparation. To analyze the data, independent samples t-test and REST (permutation test) were employed. 
Results: There was a significant increase in NHE1 mRNA in training group by 80% in comparison with the control group (P<0.05). Also, NBC1 mRNA expression was increased in trained group by 50% but it was not statistically significant at (P<0.05).
Conclusions: In conclusion, these result show that endurance training increases NHE1 NBC1 mRNA expression in myocardial in trained group and this pattern of increase in gene expression depends on transporter-type specifications that are metabolically important in intracellular pH regulation of the heart muscle.