Journal of Sports and Biomotor Sciences

Journal of Sports and Biomotor Sciences

The effect of continuous training and caloric restriction on isoprostane 8, malondialdehyde and interleukin-6 in obese male rats

Document Type : Original Article

Authors
hossien amiri 1
Sedigheh Hosseinpour Delavar 2
hasan safikhani 3
Ali Zabet 4
mahdi Beigzadeh 5
1 PhD Student, Department of Exercise Physiology, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran
2 Assistant Professor, Department of Exercise Physiology, Faculty of Litirature and Humanities, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran.
3 Assistant Professor, Department of Corrective Movements, Faculty of Litirature and Humanities, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran
4 Assistant Professor, Department of Exercise Physiology, Kangavar Branch, Islamic Azad University, Kangavar, Iran
5 Assistant Professor, Department of Exercise Physiology, West Islamabad Branch, Islamic Azad University, West Islamabad, Iran
10.22034/sbs.2025.459679.1099
Abstract
Introduction and Purpose: Obesity-induced inflammation affects various body systems. This study aimed to examine the effects of continuous exercise and calorie restriction on 8-isoprostane, malondialdehyde (MDA), and interleukin-6 (IL-6) in obese male rats.
Materials and methods: n this experimental study, 24 male rats were randomly assigned to four groups: control, exercise, calorie restriction, and exercise plus calorie restriction. The continuous exercise program lasted eight weeks, with an intensity of 24–33 meters per minute at a 15% incline. The duration increased from 10 minutes on the first day to 60 minutes per day by the fifth week and was maintained thereafter. The calorie-restricted groups received 50% of the food intake of the other groups. One-way ANOVA and Tukey's post-hoc test were used, with a significance level set at P<0.05.
Results: Levels of 8-isoprostane significantly decreased in the exercise, calorie restriction, and exercise plus calorie restriction groups compared to the control group (P<0.05). A significant difference was also observed between the exercise plus calorie restriction group and the calorie restriction group. MDA and IL-6 levels significantly decreased in the exercise, calorie restriction, and exercise plus calorie restriction groups compared to the control group (P<0.05).
Discussion and Conclusion: Eight weeks of continuous exercise combined with calorie restriction can improve levels of 8-isoprostane, MDA, and IL-6. The combination of exercise and calorie restriction is more effective than exercise without calorie restriction.
Keywords
Continuous exercise
Calorie restriction
8-isoprostane
Malondialdehyde
Interleukin-6

Subjects

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Journal of Sports and Biomotor Sciences
Volume 16, Issue 32
March 2025
Pages 83-94

  • Receive Date 27 May 2024
  • Revise Date 02 February 2025
  • Accept Date 16 February 2025